Evaluation of Vaccine-Induced Immunity Against Bacterial Infection in a Mouse Model

Abstract

Source: Caution, K., et al. Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice. J. Vis. Exp. (2019).

In this video, we describe a protocol to test the efficacy of an acellular pertussis vaccine against Bordetella pertussis infection in mice. Post-vaccination, the mice were subjected to infection, and their tissue homogenate was cultured to analyze the bacterial colonies as a readout of the vaccine-induced protective immunity.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Immunization of Mice

1. Prepare a master vaccine mix in a sterile physiological buffer such as saline or DPS.
   NOTE: The total volume in the mix should include 10% more than that required for immunization of the whole group. Concentrations of the vaccine composition are detailed below in steps 1.1.1 and 1.1.2. Transport the items to the vivarium on ice.
   1. For B. pertussis studies, include the following vaccine groups: acellular ....

Results

Figure 1: Anesthesia machine setup. Shown is an anesthetic vaporizer with an attached induction chamber and scavenger system (inside the biological safety cabinet).

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
2L induction chamber	Vet Equip	941444
Fluriso	Vet One	V1 501017	Any brand is appropriate
Casein	Sigma	C-7078
Bordet Gengou Agar Base	BD bioscience	248200
Instruments - scissors, curve scissors, forceps, fine forceps, triangle spreaders			Any brand is appropriate
15mL dounce tissue grinder	Wheaton	357544	Any similar brand is appropriate
Cordless Hand Homogenizer	Kontes/Sigma	Z359971-1EA	Any similar brand is appropriate
3mL syringes	BD bioscience	309657
15mL conical tubes	Fisher	339651
1.5mL microfuge tubes	Denville	C2170
1mL insulin syringe 28G1/2	Fisher Scientific/Excel Int.	14-841-31