Streptavidin Enrichment
Streptavidin Enrichment - Related Videos
03:41
Analyzing Influenza Virus Internalization Using a Streptavidin Blocking Assay
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2026
Source: Pohl, M. O. & Stertz, S. Measuring Attachment and Internalization of Influenza A Virus in A549 Cells by Flow Cytometry. J. Vis. Exp. (2015)This video demonstrates the use of a streptavidin blocking assay to distinguish surface-bound from internalized influenza virus in human lung epithelial cells. Comparing fluorescence signals shows that a higher intensity after incubation indicates successful viral internalization.
01:34
Environmental Enrichment for Rodents
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Environmental enrichment involves objects and practices that encourage natural behaviors, improve psychological well-being, and promote better welfare in laboratory rodents. Environmental enrichment should be tailored to the...
01:27
Environmental Enrichment in Rabbits and Pigs
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Importance of Environmental Enrichment Environmental enrichment is a fundamental component of laboratory animal care and welfare. It provides animals with opportunities to express species-specific behaviors, reduces stress, and...
09:30
A Protocol for Housing Mice in an Enriched Environment
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Cited by 70 •
2015
Environmental enrichment for mice requires a complex and challenging setup, as well as comprehensive husbandry and handling techniques to assure robust metabolic and anti-cancer effects in the mice. This protocol provides detailed procedures to reproduce the above mentioned effects in mice.
10:44
Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow
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Cited by 1 •
2025
Here, we present a protocol to enable whole-genome sequencing of bacterial sexually transmitted infections from clinical samples using target enrichment. This novel, syndromic panel-based method overcomes challenges of abundant human DNA and low bacterial loads, facilitating genomic surveillance for Chlamydia trachomatis, Neisseria gonorrhoeae, Treponema pallidum, and Mycoplasma genitalium.
FAQs
Selectivity comes from the highly specific streptavidin-biotin interaction, which allows labeled molecules to be retained while many other sample components remain unbound. This contrast is important in complex biochemical mixtures: the affinity pair provides the recognition step, and subsequent washing improves enrichment by removing material that did not associate with the immobilized streptavidin.
Immobilization converts the binding interaction into a separable capture system. Magnetic beads permit the bound fraction to be collected through magnetic separation, whereas agarose beads provide another solid support for retaining the complex during washing and recovery. The support therefore connects molecular recognition with practical handling of the sample and helps prepare material for downstream analysis.
Washing is a selectivity-control step rather than a passive handling detail. After capture, it removes unbound components from the complex sample, leaving the labeled material associated with the support. Recovery then provides the enriched fraction for analysis. Keeping these stages conceptually distinct helps explain how sample complexity is reduced without changing the target's labeling basis.
The workflow begins with a sample containing biotin-labeled target molecules and streptavidin immobilized on magnetic or agarose beads. The target is captured by the affinity interaction, unbound components are removed through washing, and the retained material is recovered. This sequence produces an enriched preparation that can move into electrophoresis, sequencing, or mass spectrometry.
In biochemistry, the method supports several distinct sample-preparation tasks: protein purification, nucleic acid isolation, immunoprecipitation, and analysis of molecular interactions. These uses share the same capture principle but differ in the material being labeled and the question being studied. The resulting enrichment can simplify complex samples before specialized analytical measurements.
Enrichment improves sample preparation by separating the biotin-labeled fraction from unbound components before analysis. The source identifies electrophoresis, sequencing, and mass spectrometry as compatible downstream techniques. Each can examine the recovered material after capture and washing, allowing the affinity step to precede sensitive biochemical or molecular measurements.