3d Printed Vessels
3d Printed Vessels - Related Videos
09:17
3D Printing of Biomolecular Models for Research and Pedagogy
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Cited by 29 •
2017
Physical models of biomolecules can facilitate an understanding of their structure-function for the researcher, aid in communication between researchers, and serve as an educational tool in pedagogical endeavors. Here, we provide detailed guidance for the 3D printing of accurate models of biomolecules using fused filament fabrication desktop 3D printers.
06:44
Ex Vivo Perfusion Culture of Large Blood Vessels in a 3D Printed Bioreactor
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Cited by 1 •
2023
This protocol presents the setup and operation of a newly developed, 3D printed bioreactor for the ex vivo culture of blood vessels in perfusion. The system is designed to be easily adopted by other users, practical, affordable, and adaptable to different experimental applications, such as basic biology and pharmacological studies.
09:38
Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method
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Cited by 6 •
2017
Scalable engineered blood vessels would improve clinical applicability. Using easily sizable 3D-printed guides, rings of vascular smooth muscle were created and stacked into a tubular form, forming a vascular graft. Grafts can be sized to meet the range of human coronary artery dimensions by simply changing the 3D-printed guide size.
11:06
3D Printing of Preclinical X-ray Computed Tomographic Data Sets
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Cited by 16 •
2013
Using modern plastic extrusion and printing technologies, it is now possible to quickly and inexpensively produce physical models of X-ray CT data taken in a laboratory. The three -dimensional printing of tomographic data is a powerful visualization, research, and educational tool that may now be accessed by the preclinical imaging community.
06:44
3D Printing - Evaluating Particle Emissions of a 3D Printing Pen
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Cited by 20 •
2020
This protocol presents a method to analyze the emission of 3D printing pens. Particle concentration and particle size distribution of the released particle is measured. Released particles are further analyzed with transmission electron microscopy (TEM). Metal content in filaments is quantified by inductively coupled plasma mass spectrometry (ICP-MS).
FAQs
Computer-designed models specify the intended vessel geometry and guide layer-by-layer deposition during fabrication. This digital control allows researchers to create tubular structures with defined shapes and channel arrangements rather than relying on uncontrolled formation. The resulting architecture provides a reproducible basis for examining fluid transport, vascular development, and cell behavior under controlled bioengineering conditions.
Sacrificial materials temporarily occupy positions where hollow channels are needed. After their removal, they leave spaces that can support fluid flow through the construct, creating a perfusable architecture. Embedded channels provide a related strategy for organizing internal pathways. These approaches are important because vascular tissue models must reproduce both tubular geometry and controlled fluid transport.
An endothelial lining is a major target because the construct must reproduce not only vessel shape but also relevant biological functions. Endothelial cells contribute to the biological interface associated with blood vessels, while the surrounding engineered structure provides physical support. Achieving an appropriate lining remains a research challenge and directly affects the prospects for tissue-engineering models and implantable grafts.
Function depends on the relationship between architecture, perfusability, biological activity, and structural durability. A construct must provide suitable channels for fluid transport, support appropriate cell behavior, and maintain enough mechanical strength for its intended use. Researchers also consider whether it can develop an endothelial lining and integrate over time, since these outcomes remain difficult to achieve together.
A typical workflow begins with a computer-designed vessel model, followed by layer-by-layer deposition of a biomaterial or cell-containing bioink. Sacrificial materials or embedded channels may then be used to establish hollow, perfusable regions. Researchers evaluate the resulting construct through its geometry, fluid transport, cell behavior, and biological performance, depending on the intended application.
These constructs are useful when researchers need a controlled platform for studying vascular development, fluid transport, or cell behavior. Their engineered geometry and material composition can support disease-modeling and drug-testing studies in a defined setting. They may also contribute to tissue engineering and the development of implantable grafts, although long-term integration and mechanical performance still require attention.
In regenerative medicine, printed vessel constructs provide a route toward engineered vascular tissues and implantable grafts. Their channels can support perfusion, while biomaterials or cell-containing bioinks help create a biologically relevant structure. The main translational obstacles are achieving sufficient mechanical strength, forming an endothelial lining, and promoting long-term integration after implantation.