Small Scale Bioreactor
Small Scale Bioreactor - Related Videos
10:49
Power Input Measurements in Stirred Bioreactors at Laboratory Scale
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Cited by 9 •
2018
The power input in stirred bioreactors can be measured through the torque that acts on the impeller shaft during rotation. This manuscript describes how an air bearing can be used to effectively reduce friction losses observed in mechanical seals and improve the accuracy of power input measurements in small-scale vessels.
12:54
Operation of a Benchtop Bioreactor
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Cited by 9 •
2013
Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture’s biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.
10:08
Construction and Setup of a Bench-scale Algal Photosynthetic Bioreactor with Temperature, Light, and pH Monitoring for Kinetic Growth Tests
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Cited by 4 •
2017
This paper describes the assembly process and operation of a bench-scale photosynthetic bioreactor that can be used, in conjunction with other methods, to estimate pertinent kinetic growth parameters. This system continuously monitors the pH, light, and temperature using sensors, a data acquisition and control unit, and open-source data acquisition software.
09:40
Batch and Continuous Bioreactors
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2023
Bioreactors are used to grow organisms in large volumes, thereby enabling the production of mass quantities of the target product. These reactors can be batch reactors, which contain all of the components needed for cell growth, or continuous reactors, which have inlet and outlet ports allowing for the addition of fresh growth media and the removal of cell waste. This video presents batch and continuous reactors and demonstrates the use of bioreactors to grow bacteria in the laboratory.
08:04
Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering
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Cited by 24 •
2013
We designed a novel mechanical loading bioreactor that can apply uniaxial or biaxial mechanical strain to a cartilage biocomposite prior to transplantation into an articular cartilage defect.
FAQs
Sensors monitor variables such as temperature, pH, and dissolved oxygen, while control systems adjust operating conditions to keep them within suitable ranges. Agitation and aeration help regulate the culture environment and support consistent nutrient and oxygen availability. This coordinated control allows researchers to examine how biological growth and product formation respond to defined process conditions.
Agitation and aeration influence how effectively oxygen and nutrients reach the cultured cells or microorganisms. Dissolved oxygen provides a measurable indicator of the available oxygen in the vessel, allowing the process to be monitored and controlled. Together, these factors help maintain conditions that support growth and can affect the formation of the desired biological product.
Its compact format allows researchers to test process conditions while using less material and laboratory space than larger systems. Results from these experiments can identify promising media, strains, cell lines, and operating conditions before larger studies begin. The resulting data help guide bioprocess optimization and provide information for planning scale-up toward biological production.
Researchers can evaluate temperature, pH, dissolved oxygen, nutrient supply, agitation, and aeration as process variables. Changing these conditions helps reveal which environment best supports cell or microorganism growth and product formation. Comparing outcomes across controlled settings supports media optimization and evaluation of strains or cell lines during early bioengineering process development.
A typical study establishes the culture conditions, supplies nutrients, and monitors variables with sensors during cultivation. Researchers then control factors such as temperature, pH, dissolved oxygen, agitation, and aeration while observing growth or product formation. They compare results among experimental conditions to identify settings that merit further optimization or scale-up.
Bioengineers use small-scale studies during early process development, especially when they need to compare media, strains, or cell lines efficiently. The reduced material and space requirements make it practical to investigate multiple controlled conditions before committing to larger equipment. Applications may include developing processes for biopharmaceuticals, enzymes, biofuels, and other biological products.