Adult Zebrafish Okr
Adult Zebrafish Okr - Related Videos
06:55
In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
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Cited by 42 •
2011
A method to conditionally knockdown a target protein’s expression in the adult zebrafish retina is described, which involves intravitreally injecting antisense morpholinos and electroporating them into the retina. The resulting protein is knocked down for several days, which allows testing the protein’s role in the regenerating or intact retina.
03:09
Gavaging Adult Zebrafish
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Cited by 80 •
2013
The increasing use of zebrafish as an animal model requires the development of effective methods for the delivery of known quantities of compounds and solutions. The gavage procedure described below allows for the oral delivery of precise volumes of solution reliably, safely and efficiently to adult zebrafish.
07:40
Dissection of the Adult Zebrafish Kidney
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Cited by 38 •
2011
The zebrafish kidney is home to both renal and hematopoietic adult stem/progenitor cells, and represents an outstanding opportunity to study these cell types and their progeny in a vertebrate model organism. Here, we demonstrate a detailed dissection procedure that enables the researcher to identify and surgically remove the adult zebrafish kidney, which can be used for applications such as cell isolation, transplantation, and expression studies of kidney and/or blood cell populations.
10:18
Dissection of Organs from the Adult Zebrafish
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Cited by 167 •
2010
This protocol describes a procedure for identifying and dissecting organs from the adult zebrafish.
08:59
Using the optokinetic response to study visual function of zebrafish
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Cited by 34 •
2010
Optokinetic response has been widely used to assess the visual functions of larval zebrafish. Nevertheless, the standard protocol for larval fish is not yet readily applicable in adults1-5. Here, we introduce how to measure the OKR of adult zebrafish using a new protocol which is established in our lab.
FAQs
The slow phase follows the direction of the moving visual pattern, while the rapid phase resets the eyes in the opposite direction. Alternation between these movements allows the assay to evaluate how effectively visual input is converted into coordinated oculomotor output. Changes in either phase can indicate altered motion processing or sensorimotor integration rather than simply reduced general activity.
OKR performance can provide information about visual acuity, motion detection, and the coordination between sensory signals and eye movements. Because the response depends on retinal stimulation and neural control of the oculomotor system, abnormal tracking may reflect changes at more than one functional level. This makes the assay useful for examining broad visual and neural phenotypes.
The response links a controlled visual stimulus to a measurable eye-movement output, creating a behavioral readout of oculomotor function. Its involuntary nature helps reduce dependence on learned task performance, allowing investigators to examine sensorimotor pathways directly through changes in tracking. In biology research, this provides a practical way to assess neural circuit function alongside visual performance.
Testing measures how the eyes respond when a moving pattern stimulates the retina. Investigators can evaluate the quality of stimulus-following movements and the accompanying resetting movements to characterize visual and oculomotor performance. The resulting behavioral profile can reveal whether a condition affects image stabilization, motion detection, or the neural coordination required for the response.
Adult zebrafish OKR offers a noninvasive approach for evaluating visual system health and neurological phenotypes. It can be applied when researchers need behavioral evidence of altered vision, motion processing, or oculomotor control without relying exclusively on anatomical measurements. The assay is therefore relevant to studies of vision disorders, neurobiology, toxicology, and drug effects.
A treatment that changes retinal function, motion detection, sensorimotor integration, or neural circuit activity may alter the measured eye-movement response. Comparing OKR performance across experimental conditions can therefore identify treatment-associated visual or neurological effects. Because the assay is noninvasive, it can serve as a behavioral outcome when assessing how compounds influence visual system health in adult zebrafish.