Anaerobic Bacteria
Anaerobic Bacteria - Related Videos
04:19
Visualization of Internalized Anaerobic Bacteria within Host Endothelial Cells
0 Views •
2025
In this video, we demonstrate a procedure to visualize internalized Porphyromonas gingivalis, an anaerobic pathogenic bacterium, within human endothelial cells using confocal microscopy. The bacteria are stained with a fluorescent dye while the host endothelial cell actin cytoskeleton is labeled with a different fluorescent dye. The internalized Porphyromonas gingivalis inside endothelial cells are visualized using confocal microscopy.
02:15
Subculturing Sulfite-Reducing Anaerobic Bacteria from an Agar Culture
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2025
Source: Anwar, Z., et al. Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples. J. Vis. Exp. (2019).In this video, sulfite-reducing bacteria are cultured in a sulfite-containing agar medium, where they grow anaerobically and reduce sulfite to hydrogen sulfide, forming black precipitates with iron ions. Black colonies are isolated from the agar depth using aseptic techniques and transferred to fresh medium for continued anaerobic incubation.
02:58
Modeling Murine Vaginal Colonization by Anaerobic Bacteria
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2025
Source: Morrill, S. R., et al. Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria. J. Vis. Exp. (2022)This video demonstrates a protocol for modeling vaginal colonization in mice using antibiotic-resistant anaerobic bacteria. It begins with hormonal treatment to increase epithelial susceptibility, followed by vaginal inoculation and co-inoculation under anaerobic conditions. The procedure concludes with post-inoculation sampling and culture to evaluate bacterial colonization...
05:28
A Bacterial Infection Assay for Studying Anaerobic Bacteria-Host Cell Interactions
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2025
This video demonstrates a technique for assessing the ability of Porphyromonas gingivalis, an anaerobic pathogenic bacterium, to adhere to and invade human umbilical vein endothelial cells (HUVECs). After infecting the host cells with the bacteria, the host cells are lysed to release both adhered and internalized bacteria. When the lysate is plated in an appropriate medium, the presence of colonies indicates successful bacteria-host cell interactions.
12:16
Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
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Cited by 17 •
2015
This article presents two protocols: one to measure anaerobic bacteria that can successfully invade and survive within the host, and the other to visualize anaerobic bacteria interacting with host cells. This study can be applied to any cultivable anaerobe and any eukaryotic cell type.
FAQs
Energy generation depends on the available electron-transfer strategy. Some anaerobic bacteria ferment, while others use nitrate or sulfate as alternative electron acceptors instead of oxygen. This distinction links their metabolism to the chemistry of oxygen-poor habitats and helps explain why different communities occupy sediments, soils, animal intestines, or infected tissues.
Obligate anaerobes may be harmed by oxygen because they lack effective defenses against reactive oxygen species, which are chemically reactive oxygen-derived compounds. Oxygen exposure can therefore restrict their persistence rather than merely reduce energy production. This vulnerability is important when interpreting their distribution in oxygen-poor tissues and other habitats with limited oxygen contact.
Fermentation and alternative-electron-acceptor metabolism provide different solutions for energy production without oxygen. Fermentation supplies energy through fermentation, whereas nitrate- or sulfate-based strategies use those substances as electron acceptors. Comparing these pathways helps connect bacterial metabolism with environmental chemistry, community composition, and the distribution of anaerobic bacteria.
Relevant sampling environments include sediments, soils, animal intestines, and infected tissues, where oxygen-poor conditions support investigation of these organisms. Comparing such settings allows researchers to relate bacterial metabolism to nutrient cycling, digestion, microbial ecology, and infection. This approach connects individual microbial lifestyles with broader biological processes across environmental and host-associated systems.
Anaerobic bacteria are relevant to wastewater treatment and biotechnology because these fields involve microbial activity under oxygen-poor conditions. Their study connects environmental management with biological energy metabolism and microbial ecology. The provided context identifies these applications but does not specify particular reactors, bacterial strains, products, or treatment performance outcomes.
Infection biology examines anaerobic bacteria because some oxygen-sensitive pathogens are associated with infected tissues. Understanding their oxygen vulnerability and energy strategies can help explain how such organisms persist in particular host environments. This subject-specific context complements their ecological importance, linking microbial physiology with the study of infections caused by oxygen-sensitive pathogens.