Chicken Egg Windowing
Chicken Egg Windowing - Related Videos
15:01
Windowing Chicken Eggs for Developmental Studies
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Cited by 37 •
2007
The ease of accessibility of the embryo has allowed for experiments to map cell fates using several approaches, including chick quail chimeras and focal dye labeling. In this article we demonstrate one egg preparation method that has been optimized for long survival times.
05:08
Egg Windowing: A Method of Opening Fertilized Chicken Egg Shell to Expose Chorioallantoic Membrane Vasculature
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2023
In this video, we describe a technique to open the eggshell of a fertilized chicken egg to expose its chorioallantoic membrane or CAM carrying a network of branching blood vessels. The exposed CAM can be used for in ovo tumor modeling.
06:56
Influenza Virus Propagation in Embryonated Chicken Eggs
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Cited by 105 •
2015
Fertile chicken eggs are widely used to produce large amounts of human influenza A virus as they provide a convenient and cost-effective system to prove high yields of virus.
03:12
Propagation and Harvest of Influenza Virus from a Fertilized Chicken Egg
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2026
Source: Brauer, R. et al., Influenza Virus Propagation in Embryonated Chicken Eggs. J. Vis. Exp. (2015)This video demonstrates the synthesis and harvest of the influenza virus in fertilized chicken eggs. The virus is inoculated into the allantoic cavity, where it replicates in epithelial cells and buds into the allantoic fluid, which is then collected and stored for downstream applications.
05:02
Purification of Virus from the Allantoic Fluid of Embryonated Chicken Eggs
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2026
Source: Yates, J. G. E., et al. Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid. J. Vis. Exp. (2022)The video demonstrates the purification and concentration of virus particles from clarified allantoic fluid collected from virus-infected embryonated chicken eggs. It showcases a series of filtration and separation steps, including depth filtration, tangential flow filtration, and density gradient ultracentrifugation to isolate intact viruses. The virus-rich...
FAQs
Retaining the embryo within the egg preserves access to its natural, nutrient-rich surroundings while development continues. This setting lets investigators follow biological changes over time rather than examining only an isolated or removed embryo. As a result, observations and interventions can be related to ongoing embryonic development, making the model useful for studying processes that unfold across multiple developmental stages.
The opening provides access, while exposing the membranes and embryo identifies the structures available for observation or manipulation. Resealing then helps maintain suitable conditions for continued incubation after access has been established. This sequence connects experimental access with preservation of the developmental setting, allowing observations or interventions without removing the embryo from the egg.
After the embryo is accessed, investigators can deliver experimental materials or manipulate developing tissues while the embryo remains in the egg. They can then collect developmental data as the process continues. This combination of intervention and follow-up is valuable for relating a treatment to later changes in organ formation, vascular development, or tissue patterning.
The technique is particularly relevant to embryology questions involving organ formation, vascular development, and tissue patterning. These areas require attention to how structures emerge and change during development. Windowing provides a way to examine those processes in a developing avian embryo and to combine visual observation with experimental manipulation or developmental data collection.
Researchers create a small opening in the shell, carefully expose the underlying membranes and embryo, perform the intended observation or manipulation, and reseal the window. The egg can then remain under continued incubation while developmental information is collected. This workflow links a temporary access point with study of the same developing embryo across subsequent stages.
Observations can document developmental changes over time, while targeted delivery or manipulation can examine how an intervention relates to development. The resulting information may concern organ formation, vascular development, tissue patterning, or other developmental data supported by the experiment. Because access occurs during incubation, researchers can connect experimental events with subsequent embryonic outcomes.
It is useful when a study needs access to a developing avian embryo but also benefits from keeping that embryo within the egg’s nutrient-rich environment. Applications include embryology, analysis of organ and vascular development, investigation of tissue patterning, and experiments requiring delivery of materials or collection of developmental data. Its value comes from combining access with continued development in place.