Colony Selection
Colony Selection - Related Videos
02:18
Isolation of Stress-Adapted Bacteria through Single Colony Selection
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2025
Source: Jeong, H., et. al. Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat. J. Vis. Exp. (2016).This video demonstrates the selective isolation and growth of stress-adapted bacteria from a culture exposed to prolonged stress conditions. The culture is plated on stressor-containing agar, and a single colony is selected to inoculate a liquid medium with the stressor. Absorbance is measured at regular intervals to monitor the growth and confirm the successful...
13:23
Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
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Cited by 13 •
2012
We present a protocol for efficient reprogramming of human somatic cells into human induced pluripotent stem cells (hiPSC) using retroviral vectors encoding Oct3/4, Sox2, Klf4 and c-myc (OSKM) and identification of correctly reprogrammed hiPSC by live staining with Tra-1-81 antibody.
07:28
Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor
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Cited by 12 •
2015
TALEN-mediated gene editing at the safe harbor AAVS1 locus enables high-efficiency transgene addition in human iPSCs. This protocol describes the procedures for preparing iPSCs for TALEN and donor vector delivery, transfecting iPSCs, and selecting and isolating iPSC clones to achieve targeted integration of a GFP gene to generate reporter lines.
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
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2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
09:04
Yeast Colony Embedding Method
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Cited by 7 •
2011
A method for embedding yeast colonies allowing sectioning for light and electron microscopy. This protocol allows determination of the distribution of sporulated cells and pseudohyphal cells within colonies providing a new tool toward understanding the organization of cell types within a fungal community.
FAQs
The antibiotic creates a selective growth condition linked to a resistance marker. Cells expressing the corresponding marker can form visible colonies, whereas organisms lacking that resistance do not appear under the stated condition. This narrows a mixed population to candidates associated with the desired selection trait, making downstream genotype confirmation more focused.
Separating colonies before culture and testing helps preserve the connection between a candidate population and its test result. A selected colony can be cultured independently, then examined by polymerase chain reaction, restriction analysis, or sequencing. This organization helps determine which isolated population carries the intended genetic change rather than combining results from multiple colonies.
Selection acts at the growth stage: the medium permits visible colony formation when organisms express the corresponding resistance marker. Screening comes afterward and asks whether an isolated colony actually has the intended genotype. Polymerase chain reaction, restriction analysis, and sequencing provide confirmation beyond growth alone, separating the ability to grow under selection from evidence of the desired genetic construct.
A practical workflow plates cells on selective medium, identifies visible colonies, and picks candidates separately. Each picked colony is cultured and then screened by polymerase chain reaction, restriction analysis, or sequencing. Comparing the resulting test information with the intended genotype identifies populations suitable for downstream cloning, recombinant protein production, gene editing, or strain-development work.
In plasmid cloning, colony selection helps identify populations associated with the desired construct before further use. The same logic supports recombinant protein production and gene-editing workflows, where researchers need cells linked to a particular genetic outcome. It also contributes to developing genetically characterized strains, providing defined populations for subsequent biological studies.
Growth under selective conditions indicates expression of a corresponding resistance marker, but it does not by itself confirm the complete intended genotype. Adding polymerase chain reaction, restriction analysis, or sequencing supplies genetic evidence. Combining these observations supports more reliable identification of strains whose selected phenotype and genetic characteristics have both been examined.