Commensalism

Commensalism is a biological interaction in which one organism benefits while the other experiences no significant benefit or harm. The relationship can arise when one species gains food, shelter, transportation, or access to resources through another without substantially affecting its partner, as when remoras attach to sharks and feed on leftover meals or epiphytic plants use trees for support. Studying commensalism helps biologists classify species interactions, understand community structure, and evaluate how organisms share habitats and resources. It also provides a framework for comparing commensalism with mutualism, in which both species benefit, and parasitism, in which one is harmed.

Commensalism - Related Videos

Research

JoVE EoE - Rodent Models

Swab-based Conjunctival Commensal Bacteria Isolation: A Technique to Isolate Commensal Bacteria from Conjunctiva of Murine Model

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2025

This video describes a swab-based technique to isolate viable commensal bacteria from the ocular conjunctiva. The technique can be used to profile the commensal bacterial microbiome of the conjunctiva, which plays a defensive role against pathogens.

Conjunctival Commensal Isolation and Identification in Mice

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2021

Presented here is a protocol for the isolation and amplification of aerobic and facultative anaerobic mouse conjunctival commensal bacteria using a unique eye swab and culture-based enrichment step with subsequent identification by microbiological based methods and MALDI-TOF mass spectrometry.

Research

JoVE Journal - Immunology and Infection
Free Sample

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

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Cited by 45 •

2019

Here, we present a protocol to culture human enteroid or colonoid monolayers that have intact barrier function to study host epithelial-microbiota interactions at the cellular and biochemical level.

Quantifying Human Norovirus Virus-like Particles Binding to Commensal Bacteria Using Flow Cytometry

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Cited by 8 •

2020

The goal of this protocol is to quantify binding of the eukaryotic pathogen human norovirus to bacteria. After performing an initial virus-bacterium attachment assay, flow cytometry is used to detect virally-bound bacteria within the population.

A Galleria mellonella Oral Administration Model to Study Commensal-Induced Innate Immune Responses

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Cited by 12 •

2019

Here, we provide a detailed protocol for an oral administration model using Galleria mellonella larvae and how to characterize induced innate immune responses. Using this protocol, researchers without practical experience will be able to use the G. mellonella force-feeding method.

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