Crispr System
Crispr System - Related Videos
01:59
CRISPR
0 Views •
2019
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
02:53
CRISPR and crRNAs
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2020
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea. The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
11:53
Substrate Generation for Endonucleases of CRISPR/Cas Systems
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Cited by 4 •
2012
CRISPR/Cas systems mediate adaptive immunity in Bacteria and Archaea. Many Cas proteins are proposed to act as endoribonucleases acting on crRNA precursors of varying length. Here we illustrate three different approaches to generate pre-crRNA substrates for the biochemical analysis of Cas endonuclease activity.
02:55
Genetically Modifying CAR T Cells Using a CRISPR-Cas9 System
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2025
The video outlines a process for creating genetically modified CAR T cells through the CRISPR-Cas9 System. Infecting T cells with CRISPR and CAR lentiviruses results in modifications to the target gene and the synthesis of a chimeric antigen receptor or CAR, ultimately leading to the formation of genetically modified CAR T cells.
04:22
A Technique for Gene Editing in Natural Killer Cells Using CRISPR Cas9
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2025
This video demonstrates a technique for Cas9 ribonucleoprotein-mediated genetic modification of primary natural killer (NK) cells. A Cas9 ribonucleoprotein, consisting of a Cas9 endonuclease bound to a guide RNA (gRNA) formed by base pairing a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA), is introduced into primary natural killer cells via electroporation. The ribonucleoprotein targets and cleaves the host DNA at the target site, leading to gene knockout via modification of the...
FAQs
Target recognition depends on two coordinated signals: guide-RNA complementarity identifies a matching nucleic-acid sequence, while a nearby protospacer-adjacent motif, or PAM, helps establish whether that sequence is eligible for recognition and cleavage. This combination gives the CRISPR-Cas complex both sequence information and a local molecular condition to evaluate when selecting a target.
Cas proteins provide the active machinery that responds to guide-RNA-directed recognition. Their activity can result in cleavage of the selected DNA or RNA sequence, while engineered CRISPR-Cas configurations can produce other outcomes, including gene modification, repression, activation, or labeling. Thus, the Cas component helps determine what biological effect follows target recognition.
In microbes, the system contributes to adaptive defense against returning viruses or other foreign nucleic acids. Biology researchers redirect the same guide-RNA and Cas-protein targeting logic toward selected genes. This engineered use shifts the emphasis from microbial defense to controlled investigation or regulation of gene sequences and their biological effects.
A high-level workflow starts by selecting a gene or nucleic-acid sequence relevant to the research question, then designing or using a guide RNA with matching complementarity and an appropriate nearby PAM. The guide is paired with a Cas protein, and the resulting activity is assessed according to whether the goal was modification, repression, activation, or labeling.
The biological question guides the desired CRISPR outcome. Researchers may seek to modify a gene to examine its consequences, repress or activate it to study regulation, or label it to track its location or behavior. Choosing among these outcomes allows the same targeting principle to address different aspects of gene function and genome regulation.
CRISPR-based experiments can connect a selected gene with its biological role by changing, reducing, increasing, or labeling its activity or presence. These outcomes support investigations of gene function and genome regulation, and they can help researchers examine how genetic changes contribute to disease mechanisms. The findings may also inform potential therapeutic strategies.
Its programmable targeting makes it useful for examining specific genes linked to biological processes or disease mechanisms. Researchers can investigate the consequences of modifying, repressing, activating, or labeling those genes rather than studying genome regulation only in general terms. Such experiments provide a research foundation for considering potential therapeutic strategies, while keeping the immediate goal focused on biological investigation.