Dna Binding Plate
Dna Binding Plate - Related Videos
05:00
Fluorescence Anisotropy to Determine Transcription Factor-DNA Binding Affinity
0 Views •
2025
This video describes high-performance fluorescence anisotropy that helps to monitor the interaction between transcription factors and DNA. The assay monitors the interactions of a fluorophore-labeled DNA molecule with its specific transcription factor by measuring the degree of polarization due to molecular rotation or anisotropy of the fluorophore-labeled DNA.
08:40
Methyl-binding DNA capture Sequencing for Patient Tissues
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Cited by 1 •
2016
Here we present a protocol to investigate genome wide DNA methylation in large scale clinical patient screening studies using the Methyl-Binding DNA Capture sequencing (MBDCap-seq or MBD-seq) technology and the subsequent bioinformatics analysis pipeline.
08:51
Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide
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Cited by 7 •
2016
We present an approach for visualizing fluorescent protein DNA binding peptide (FP-DBP)-stained large DNA molecules tethered on the polyethylene glycol (PEG) and avidin-coated glass surface and stretched with microfluidic shear flows.
07:05
Chromatin Immunoprecipitation to Identify Target Protein Binding Sites on Genomic DNA
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2025
In this video, we demonstrate the chromatin immunoprecipitation technique to identify protein binding sites on specific regions of the genomic DNA of oligodendrocyte precursor cells via the selective immunoprecipitation of protein-bound chromatin fragments. This method helps to study the interaction of several regulatory proteins, including transcription factors involved in gene regulation.
01:03
Single-Strand DNA Binding Proteins
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2023
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
FAQs
Binding depends on chemical conditions that promote interaction between DNA and the plate’s silica-based surface. Chaotropic salts and high alcohol concentrations create the binding environment, allowing DNA to be retained while the sample passes through the well. This selective capture is the central separation step before washing and elution.
Chaotropic salts and alcohol do more than simply prepare the sample: their concentrations determine whether DNA remains associated with the silica surface. Under the high-salt, high-alcohol conditions described for binding, proteins and other contaminants can be removed during washing. This chemical selectivity helps separate DNA from unwanted sample components before the purified material is recovered.
Elution is driven by lowering the salt concentration after the washing stage. Changing this condition weakens the interaction that held DNA on the silica-based membrane or surface, allowing the nucleic acid to leave the plate and enter the recovered liquid. The resulting eluate supplies DNA for downstream molecular biology procedures rather than leaving it immobilized in the well.
A typical plate-based workflow moves through binding, washing, and elution in sequence. A biological sample is exposed to chaotropic salts and high alcohol concentrations so DNA binds, contaminants are removed during washing, and a lower-salt condition releases the DNA. Running these operations across multiple wells allows samples to be processed in parallel within one purification workflow.
Researchers can use this format when many samples require DNA purification for PCR, genotyping, sequencing, or cloning. The multiwell design supports parallel handling instead of requiring each sample to be processed as a separate workflow, which can improve efficiency and consistency. Its value is greatest when the same capture, wash, and recovery sequence must be applied across a sample set.
Parallel well processing can make the purification sequence more consistent across a group of samples. Each well can undergo the same binding, washing, and elution logic, while the plate format reduces the need to repeat the workflow separately for every sample. This supports efficient preparation of DNA for downstream molecular biology analyses.