Giga Seal
Giga Seal - Related Videos
04:41
Optimized Sealing Process and Real-Time Monitoring of Glass-to-Metal Seal Structures
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Cited by 7 •
2019
Key procedures to optimize the sealing process and achieve real-time monitoring of the metal-to-glass seal (MTGS) structure are described in detail. The embedded fiber Bragg grating (FBG) sensor is designed to achieve online monitoring of temperature and high-level residual stress in the MTGS with simultaneous environmental pressure monitoring.
07:51
The C-seal: A Biofragmentable Drain Protecting the Stapled Colorectal Anastomosis from Leakage
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Cited by 8 •
2010
The C-seal is a biofragmentable drain protecting the stapled colorectal anastomosis. In this video, details of the manufacturing, deployment and use are shown.
06:10
Accessible Silicone Chip-to-Membrane Sealing Procedure for Flexible, Reliable Bonding
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2026
A novel sealing protocol to produce water-tight silicone-based seals for membranes of Polydimethylsiloxane (PDMS) chip devices. Intended for labs setting-up chip models and small-scale chip platform development. We demonstrate the protocol using plastic and native tissue membranes. This procedure only requires PDMS, toluene, mold, membrane, and a vacuum chamber.
06:26
Achieving Moderate Pressures in Sealed Vessels Using Dry Ice As a Solid CO2 Source
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Cited by 11 •
2018
Here we present a protocol for performing reactions in simple reaction vessels under low-to-moderate pressures of CO2. The reactions can be performed in a variety of vessels simply by administering the carbon dioxide in the form of dry ice, without the need for costly or elaborate equipment or set-ups.
04:36
Vacuum-Sealed Hot Water Bath Immersion for the Preparation of Anatomical and Surgical Cadaveric Bone Models
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Cited by 1 •
2022
The present protocol describes the maceration and cleaning of cadaveric bone with a vacuum-sealed, hot water bath immersion technique. This is a low-cost, safe, and effective method to produce anatomical specimens for surgical planning and medical education as an alternative to three-dimensional (3D) printed models.
FAQs
Its high resistance restricts unwanted ionic leakage at the pipette-membrane interface. By reducing current that bypasses the membrane region being studied, the seal also lowers electrical noise. This improved isolation allows measured signals to more closely represent membrane potential, whole-cell currents, or currents passing through individual ion channels rather than leakage around the pipette.
Gentle suction helps bring the cell membrane into close contact with the glass pipette. The resulting apposition between the glass surface and lipid bilayer creates the high-resistance interface required for low-leakage recording. Both actions are therefore central to forming the electrical barrier, rather than serving merely as mechanical steps for positioning the pipette.
A resistance above one gigaohm indicates that the pipette-membrane interface strongly limits unintended current flow. This matters because leakage can obscure the small electrical signals generated by cells or individual ion channels. Achieving this level of resistance supports cleaner measurements and helps researchers distinguish physiological membrane activity from current escaping through the recording interface.
After the seal forms, the recording configuration can support several measurements described in patch-clamp experiments. Researchers may monitor membrane potential, record currents across the whole cell, or examine currents through individual ion channels. These outcomes make the seal useful for connecting electrical recordings with cellular physiology at either the whole-cell or single-channel level.
The pipette is brought into contact with the cell membrane, followed by application of gentle suction. Close apposition between the glass and lipid bilayer then produces the high-resistance interface needed for recording. The procedure is successful when the resulting seal provides the low-leakage electrical connection required to measure membrane signals with reduced noise.
This approach is especially relevant when investigators need direct electrical information from neurons, other excitable cells, or cells whose ion-channel activity is under study. It supports research on neuronal signaling, channel function, and cellular physiology by providing measurements of membrane potential or ionic currents at whole-cell and individual-channel scales.