Glucocorticoid Receptor
Glucocorticoid Receptor - Related Videos
08:02
Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
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Cited by 6 •
2016
Adrenal activity can be assessed in the equine species by analysis of feces for corticosterone metabolites. The method offers a non-invasive option to assess long term patterns in both domestic and free ranging horses. This protocol describes the enzyme linked immunoassay involved and the associated biochemical validation.
01:25
Antiasthma Drugs: Inhaled Corticosteroids and Glucocorticoids
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2024
Inhaled corticosteroids (ICS) are anti-inflammatory drugs used primarily in treating persistent asthma and providing long-term maintenance. They target the bronchial mucosa, the lining of the airways, to control inflammation, a critical factor in asthma progression and exacerbation. ICS work through a multifaceted mechanism of action. They suppress the inflammatory response caused by the proliferation of TH cells. They also reduce the transcription of the IL-2 gene, which is involved in the...
11:07
Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
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Cited by 1 •
2011
An in vitro method for preparing functional glucocorticoid receptor (GR)•hsp90 protein complexes from purified proteins and cellular lysates is described. The method utilizes immunoadsorption of recombinant GR followed by salt-stripping and protein complex reconstitution. The importance of cofactors and buffer conditions are discussed, as are potential method applications.
01:21
Drugs for Treatment of Crohn's Disease in IBD Using Glucocorticoids
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2024
Glucocorticoids, a class of anti-inflammatory drugs, are pivotal in treating moderate to severe Crohn's disease by inducing remission. They exhibit their anti-inflammatory action by inhibiting the production of inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1, and chemokines like IL-8. In addition, they reduce the expression of inflammatory cell adhesion molecules and inhibit gene transcription of nitric oxide synthase, phospholipase A2, cyclooxygenase-2 (COX-2),...
12:03
Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
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Cited by 14 •
2016
Here we present a protocol to describe the localization of angiotensin II Type 1 receptors in the rat brain by quantitative, densitometric, in vitro receptor autoradiography using an iodine-125 labeled analog of angiotensin II.
FAQs
Chaperone association helps control the receptor’s state before hormone binding. When cortisol or another steroid hormone binds, the receptor changes conformation, a structural shift that enables its relocation from the cytoplasm to the nucleus. This transition links hormone detection to the transcriptional events that ultimately alter cellular behavior.
The receptor can influence transcription through two related nuclear interactions: binding glucocorticoid response elements and associating with other transcriptional regulators. These routes provide a molecular connection between receptor activation and changes in gene expression, allowing hormone signaling to affect programs linked to inflammation, development, and cellular homeostasis.
Its importance extends beyond immediate stress responses because receptor-regulated genes participate in multiple aspects of cell and organismal function. Changes in these genes can influence metabolism, immune activity, development, and cellular homeostasis. This broad transcriptional reach explains why the receptor connects endocrine signals with diverse biological outcomes.
Researchers can examine hormone-dependent changes in receptor location, its movement from the cytoplasm into the nucleus, and its interaction with response elements or other transcriptional regulators. They can then relate these signaling events to altered expression of genes involved in inflammation, development, and homeostasis, supporting investigations of endocrine regulation and immune disease.
Drugs that target the glucocorticoid receptor remain important anti-inflammatory therapies because this receptor controls gene-expression programs associated with immune activity and inflammation. Studying how receptor signaling changes transcription helps place these therapies within the broader biology of endocrine regulation and provides context for research on immune disease.
Steroid hormones such as cortisol use the receptor to translate stress-related hormonal signals into changes in nuclear gene regulation. The resulting effects can extend across metabolism and immune activity, while also influencing cellular homeostasis. This connection makes the receptor a central subject in biology research on stress responses and endocrine control.