Hema Cytometer
Hema Cytometer - Related Videos
10:19
Using a Hemacytometer to Count Cells
0 Views •
2023
Many biomedical experiments require manipulation of a known quantity of cells, in order to achieve accurate, reproducible, and statistically-relevant data. Therefore, learning how to count cells is a particularly essential technique for any successful biomedical scientist. The most common way to count cells is by using a hemacytometer - an instrument that bears two laser-etched grids, which aid in the enumeration of an aliquot cells under a simple light microscope. This data can then be used to...
09:37
Isolation and Expansion of Adult Canine Hippocampal Neural Precursors
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Cited by 3 •
2016
The canine brain is a valuable model in which to study adult neurogenesis. Presented here are protocols for isolating and expanding adult canine hippocampal neural precursor cells from primary brain tissue.
08:23
Phenotypic Analysis of Rodent Malaria Parasite Asexual and Sexual Blood Stages and Mosquito Stages
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Cited by 10 •
2019
Due to the striking similarities of the life cycle and biology of rodent malaria parasites to human malaria parasites, rodent malaria models have become indispensable for malaria research. Herein, we standardized some of the most important techniques used in the phenotypic analysis of wild-type and transgenic rodent malaria species.
10:48
Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
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Cited by 8 •
2017
Robust derivation of human induced pluripotent stem (hiPS) cells was achieved by using non-integrating Sendai virus (SeV) vector mediated reprogramming of dermal fibroblasts. hiPS cell maintenance and clonal expansion was performed using xeno-free and chemically defined culture conditions with recombinant human laminin 521 (LN-521) matrix and Essential E8 (E8) Medium.
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
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2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
FAQs
A Hema Cytometer tracks cells individually as they pass through the analyzer. Detection relies on measurable physical signals, including cell size, electrical resistance, or light-scattering behavior. These signals allow the system to separate cellular events and quantify the corresponding populations. This individual-event approach supports consistent assessment of blood composition.
Each physical property provides a basis for distinguishing cells during measurement. Differences in size, electrical resistance, or light-scattering behavior help the analyzer assign detected events to cellular populations rather than treating the sample as an undifferentiated mixture. Using these measurable characteristics supports quantitative characterization of red blood cells, white blood cells, and platelets.
Standardized counting makes results more comparable across samples and experimental runs. Because the analyzer produces quantitative measurements of cellular populations and concentrations, researchers can assess changes using a consistent measurement approach. This improves workflow efficiency and reproducibility, which is especially valuable when investigating biological variation, disease-related alterations, or responses to experimental treatments.
Measurements can reveal shifts affecting red blood cells, white blood cells, and platelets. Examining these populations provides quantitative information about changes in hematological composition rather than relying only on general observations. Such results can help researchers investigate altered physiology, identify disease-related cellular changes, and examine how experimental conditions influence blood-cell populations.
The workflow begins when a blood sample passes through the analyzer, where cells are detected individually. The instrument evaluates physical properties associated with those events, then produces quantitative information about cellular populations and concentration. Researchers can use the resulting measurements to assess hematological composition and compare samples within a biological or clinical research workflow.
The measurements provide quantitative information about blood-cell populations and their concentration. Results can be considered across red blood cells, white blood cells, and platelets to identify changes in hematological composition. Because the output is numerical and standardized, it supports comparisons among samples and helps researchers evaluate whether cellular patterns change under different biological or experimental conditions.
Biology researchers may use the instrument to assess normal physiology, investigate disease-related alterations, or evaluate experimental treatments. It can also help monitor cellular responses during a study by providing repeated, quantitative measurements of blood-cell populations. These applications make the technique relevant whenever changes in hematological composition are an important experimental outcome.
By measuring blood-cell populations and concentrations in a standardized way, the instrument can provide quantitative evidence of changes associated with an experimental treatment. Researchers can compare hematological composition before or across study conditions and monitor cellular responses. The resulting data support assessment of treatment-related effects while improving the efficiency and reproducibility of the research workflow.