High-density Colony Arrays
High-density Colony Arrays - Related Videos
02:25
Photodegradable Hydrogel-Based Isolation of Bacterial Colonies in Microwell Arrays
0 Views •
2025
Source: Fattahi, N., et al. Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation. J. Vis. Exp. (2021)This video demonstrates the use of photodegradable hydrogels to isolate bacterial colonies within silicon microwell arrays. Bacterial cells are first loaded into microwells, encapsulated in a crosslinked hydrogel, and cultured into distinct colonies. Upon exposure to ultraviolet light, targeted degradation of the hydrogel enables selective extraction of individual...
12:15
Growth Curves: Generating Growth Curves Using Colony Forming Units and Optical Density Measurements
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2023
Source: Andrew J. Van Alst1, Rhiannon M. LeVeque1, Natalia Martin1, and Victor J. DiRita1 1 Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America Growth curves provide valuable information on bacterial growth kinetics and cell physiology. They allow us to determine how bacteria respond in variable growth conditions as well as to define optimal growth parameters for a given bacterium. An archetypal growth curve progresses...
09:00
Investigating The Network-Wide Mechanisms of Pallidal Deep Brain Stimulation Using High-Density Microelectrode Arrays
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2026
Here, we describe a protocol for ex vivo high-density microelectrode array recordings from acute cerebellar brain slices of a dystonic hamster model that received in vivo. continuous pallidal deep brain stimulation for 11 days.
08:07
Ultrahigh Density Array of Vertically Aligned Small-molecular Organic Nanowires on Arbitrary Substrates
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Cited by 3 •
2013
We report a simple method for fabricating an ultrahigh density array of vertically ordered small-molecular organic nanowires. This method allows for synthesis of complex heterostructured hybrid nanowire geometries, which can be inexpensively grown on arbitrary substrates. These structures have potential applications in organic electronics, optoelectronics, chemical sensing, photovoltaics and spintronics.
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
0 Views •
2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
FAQs
Each position serves as a location tied to a particular sample, allowing researchers to track that sample throughout growth and analysis. Because neighboring colonies remain spatially separated, observed differences can be assigned to defined samples rather than treated as an undifferentiated mixture. This organization is especially valuable when many variants or strains must be compared in parallel.
Controlled growth conditions allow deposited cells to expand from a small starting population while preserving the array’s planned organization. Consistent conditions make colony development more comparable across positions, which supports interpretation of differences in colony phenotypes. This matters for screening and strain comparison, where the observed outcome must remain connected to the sample location.
The address of a colony provides the link between an observable phenotype and the corresponding biological sample or genetic variant. After growth, researchers can examine differences among positions, identify samples associated with traits of interest, and recover selected colonies. This traceability turns a spatial pattern into information about the underlying collection, supporting library characterization and selection.
A typical workflow begins by depositing or transferring cells into defined positions on a solid surface. The array is then grown under controlled conditions so each colony expands from a small population while remaining identifiable. Researchers can analyze colony phenotypes, compare samples or variants, and recover colonies associated with a desired result. The ordered positions preserve sample identity throughout these steps.
They are suited to experiments that must evaluate many related biological samples in parallel, including high-throughput genetic screening, strain comparison, and library characterization. The format also supports selection of organisms with desired traits because researchers can associate a position and its colony phenotype with a particular sample. These uses make arrays valuable when sample identity and parallel processing are both important.
Array results can reveal differences in colony phenotypes across strains, genetic variants, or library members. Since each observation remains tied to an addressable position, researchers can identify which sample produced a phenotype of interest and recover that colony for further use. These outcomes support comparison, characterization, and selection while preserving the connection between phenotype and sample identity.