Male Sterile Mutants
Male Sterile Mutants - Related Videos
14:45
Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency
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Cited by 28 •
2014
De novo mutations in the male germline may contribute to adverse health outcomes in subsequent generations. Here we describe a protocol for the use of a transgenic rodent model for quantifying mutations in male germ cells induced by environmental agents.
12:29
Sterile Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research. The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting...
02:21
Enriching for Males: Maintaining a Stock of Male C. elegans
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2023
Nematodes can be propagated easily in the lab--in part because hermaphrodites can reproduce by self-fertilization. However, genetic crosses and other experiments may require a large number of males. This video describes a method to enrich for male C. elegans.
08:58
Preparing Irradiated and Marked Male Aedes aegypti Mosquitoes for Release in an Operational Sterile Insect Technique Program
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Cited by 5 •
2021
The sterile insect technique (SIT) is used to control specific, medically important mosquito populations that may be resistant to chemical controls. Here, we describe a method of mass rearing and preparation of sterile male mosquitoes for release in an operational SIT program targeting the Aedes aegypti mosquito.
09:17
Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish
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2025
Here, we describe a protocol for generating maternal mutant that couples a stable zpc:cas9 knock-in line with Tol2-mediated delivery of sgRNA expression cassettes.
FAQs
The genetic effect can appear at several checkpoints in male reproductive development. A mutation may interfere with anther formation, the meiotic divisions that generate reproductive cells, pollen development, or final pollen maturation. Identifying the affected stage helps connect a visible fertility phenotype with a particular developmental process and can guide studies of the responsible gene.
Because the mutation described affects male gamete production or release, it does not necessarily eliminate the organism’s ability to receive pollen and support the female side of reproduction. This separation lets investigators use the mutant as a maternal parent in crosses, while another plant supplies the pollen needed for fertilization.
Male sterile mutants provide a genetic route to preventing self-fertilization, whereas manual anther removal physically eliminates the male structures from individual flowers. The mutant approach can reduce the need for repeated hand removal during crossing. It also gives biology researchers a way to investigate which developmental or fertility-regulation processes are disrupted.
Researchers use a male sterile plant as the parent that receives pollen and apply pollen from a selected second plant. Because the mutant cannot provide functional male gametes, the transferred pollen supplies the male contribution. The resulting cross combines the reproductive roles of the two parents and supports deliberate study or production of offspring.
By limiting the mutant plant’s contribution to the female side, breeders can combine it with pollen from a chosen parent rather than relying on self-fertilization. This helps generate hybrid seed with a more predictable parental combination. The practical advantage is reduced manual anther removal, making planned crosses more manageable in crop-breeding work.
These mutants provide experimental material for linking genes to reproductive development and fertility regulation. Researchers can ask whether a candidate gene affects anther formation, meiosis, pollen development, or maturation by examining the stage at which fertility fails. The same material therefore connects genetic change with reproductive phenotype and with broader studies of plant development.