Microfluidic Gas Network
Microfluidic Gas Network - Related Videos
10:53
Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
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Cited by 10 •
2017
This protocol outlines the implementation of image-guided, laser-based hydrogel degradation to fabricate vascular-derived, biomimetic microfluidic networks embedded in poly(ethylene glycol) diacrylate (PEGDA) hydrogels. These biomimetic microfluidic systems may be useful for tissue engineering applications, generation of in vitro disease models, and fabrication of advanced "on-a-chip" devices.
27:58
Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber
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Cited by 8 •
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We demonstrate fabrication of a simple microfluidic device that can be integrated with standard electrophysiology setups to expose microscale surfaces of a brain slice in a well controlled manner to different neurotransmitters.
10:35
Studies of Bacterial Chemotaxis Using Microfluidics - Interview
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Cited by 1 •
2007
05:41
Applying Microfluidics to Electrophysiology
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Cited by 1 •
2007
Microfluidics can be integrated with standard electrophysiology techniques to allow new experimental modalities. Specifically, the motivation for the ...
07:51
Gas Absorber
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2023
Source: Michael G. Benton and Kerry M. Dooley, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA Gas absorbers are used to remove contaminants from gas streams. Multiple designs are used to accomplish this objective1. A packed bed column uses gas and liquid streams running counter to each other in a column packed with loose packing materials, such as ceramics, metals, and plastics, or structured packing1. The packed bed uses surface area created by the packing to...
FAQs
Gas moves because of pressure differences between connected regions, while channel resistance limits how readily it can pass through each pathway. Changing either factor alters the distribution and delivery rate across the network. This relationship allows researchers to regulate gas exposure in microchambers rather than relying on uncontrolled movement through the biological environment.
These components control how gas travels through interconnected channels. Valves can direct gas along selected pathways, membranes can help regulate delivery between regions, and flow restrictors limit passage through a channel. Together, they provide finer control over which microchambers receive a gas and how the surrounding biological conditions are maintained.
Small volumes support rapid changes in the local gas environment and allow exposure to remain spatially focused. A researcher can therefore examine how cells, microorganisms, or tissue models respond to altered conditions in a confined region. This localized control is particularly relevant when studying transitions in oxygen, carbon dioxide, humidity, or other gases.
By regulating oxygen, carbon dioxide, humidity, or other gases near a sample, the system can reproduce selected environmental conditions without changing the entire experimental setting. Such control helps connect gas exposure with biological behavior, including cellular respiration, microbial growth, gas exchange, and responses associated with reduced oxygen availability.
A basic workflow begins by establishing pressure differences across the network, then directing gas through channels with valves, membranes, or flow restrictors. The regulated stream reaches designated microchambers containing cells or tissue models. Researchers can then examine responses to the resulting local gas conditions, including changes related to respiration, hypoxia, or gas exchange.
This approach is suited to questions about how biological systems respond to controlled gas environments. Applications include examining cellular respiration, modeling hypoxia, investigating gas exchange, assessing microbial growth, and exposing tissue models to physiologically relevant conditions. The network is especially useful when the experiment requires localized exposure or rapid adjustment of the surrounding atmosphere.
Controlling particular gases allows researchers to relate biological outcomes to defined environmental changes rather than to broadly altered culture conditions. Oxygen and carbon dioxide can support studies of respiration, hypoxia, or gas exchange, while humidity provides another regulated variable. Applying these conditions around microorganisms, cells, or tissue models helps reveal responses to their local environment.