Mouse Calvaria
Mouse Calvaria - Related Videos
03:15
Calvarial Window Generation in Mouse Model: A Surgical Procedure to Create Window in the Top Part of the Mouse Skull
0 Views •
2023
In this video, we describe the surgical procedure to create a calvarial window for accessing the brain of a mouse model.
08:24
Measuring Bone Remodeling and Recreating the Tumor-Bone Microenvironment Using Calvaria Co-culture and Histomorphometry
0 Views •
Cited by 3 •
2020
The ex vivo culture of bone explants can be a valuable tool for the study of bone physiology and the potential evaluation of drugs in bone remodeling and bone diseases. The presented protocol describes the preparation and culture of calvarias isolated from newborn mice skulls, as well as its applications.
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
0 Views •
Cited by 59 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
07:04
A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse
0 Views •
Cited by 5 •
2017
Mice are widely used to study gestational biology. However, pregnancy termination is required for such studies which precludes longitudinal investigations and necessitates the use of large numbers of animals. Therefore, we describe a non-invasive technique of high-frequency ultrasonography for early detection and monitoring of post-implantation events in the pregnant mouse.
07:46
In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
0 Views •
Cited by 39 •
2010
Orthotopic animal models of ovarian cancer replicate better human disease and therefore enhance our understanding of cancer progression and tumor response to therapy. A mouse model receives an intrabursal injection of luciferase-expressing ovarian tumor cells. Treatment is administered via oral gavage. Tumor growth is monitored by in vivo imaging system.
FAQs
Their development follows a relatively well-characterized pathway of intramembranous ossification, allowing researchers to examine how mesenchymal cells become osteoblasts and how those cells build mineralized extracellular matrix. The tissues also support controlled ex vivo cultures and in vivo analyses, so investigators can connect cellular events with changes in bone formation, regeneration, or remodeling.
Mesenchymal cells first differentiate into osteoblasts, the bone-forming cells responsible for producing and mineralizing extracellular matrix. This pathway forms the calvarial tissue without the developmental sequence represented by other types of bone formation. Studying it helps researchers isolate mechanisms that regulate osteoblast differentiation and matrix mineralization during skull-vault development.
Osteoblasts establish new bone by producing and mineralizing extracellular matrix, whereas osteoclasts participate later in remodeling the tissue. Examining both cell populations is important because bone biology depends on formation followed by controlled tissue modification. Mouse calvaria therefore provide a setting for relating osteoblast activity and osteoclast-mediated remodeling to skeletal development and repair.
Ex vivo cultures provide a controlled setting for examining cellular responses and testing osteogenic factors or biomaterials, while in vivo analyses reveal how those responses occur within developing or remodeling tissue. Using both approaches helps distinguish direct cellular effects from outcomes that depend on the broader biological environment, strengthening interpretation of bone-regeneration studies.
Calvarial tissues and cells can be examined after exposure to biomaterials or osteogenic factors in controlled experimental systems. Researchers assess how these interventions influence bone-forming processes and tissue responses, then relate the findings to regeneration. This application makes the model relevant for evaluating strategies intended to support craniofacial repair or address broader skeletal problems.
The model supports investigations of how inflammation affects bone biology and how biological interventions may promote repair. Researchers can connect cellular mechanisms of formation and remodeling with outcomes relevant to craniofacial tissues. Findings may also provide context for broader skeletal disorders, particularly when ex vivo observations are considered alongside in vivo analyses.