Mouse Head Bisecting
Mouse Head Bisecting - Related Videos
04:13
Modeling Repetitive Concussive Head Injuries in a Mouse Model
0 Views •
2025
Source: Yang, Z., et.al. A Repetitive Concussive Head Injury Model in Mice. J. Vis. Exp. (2016).This video demonstrates the induction of repetitive concussive head injuries in a mouse using an electromagnetic impact device. The procedure involves positioning the impact tip between the interfrontal and lambdoid sutures on the skull of an anesthetized mouse and using a probe tip to precisely set the impact depth. The impact is delivered at a controlled velocity and depth to simulate mild...
02:34
Mouse Headpost Implantation: A Method for Head Fixation in Rodents
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2023
This video shows a headpost implantation surgery in murine model. The headpost is used not only to head-fix and therefore restrain the mouse during experimentation, but also to securely attach a head device in freely moving animals.
06:15
Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
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Cited by 3 •
2022
Here, we present a protocol to significantly reduce the mitochondrial DNA copy numbers in a bovine oocyte (P < 0.0001). This method utilizes centrifugation and bisection to substantially reduce oocyte mitochondria and may allow for an increased chance of development in the reconstructed interspecies somatic cell nuclear transfer embryos.
08:59
A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma
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Cited by 21 •
2017
Perineural invasion (PNI) is a common feature of head and neck squamous cell carcinoma (HNSCC), conferring lower survival rates. Its mechanisms are poorly understood. Utilizing neurites generated from murine dorsal root ganglia confined to a semisolid matrix, the pathways involved in the PNI of HNSCC cell lines can be investigated.
01:19
Muscles that Move the Head
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2024
The muscles that move the head are a dynamic and complex group of structures that work together to facilitate a wide range of head movements, including rotation, flexion, extension, and lateral bending. The bilateral sternocleidomastoid, or SCM, and the suprahyoid and infrahyoid muscles are significant head flexors. The SCM muscles originate at the sternum and clavicle and attach to the mastoid process of the temporal bone. The SCM contracts bilaterally to bend the head forward, whereas...
FAQs
The anatomical plane determines which structures can be compared between the two resulting sections. A midsagittal cut follows the head’s midline, producing left and right portions that retain corresponding neural, sensory, dental, and facial regions in a consistent orientation. That consistency is important when examining organization, pathology, developmental changes, or treatment effects across the specimen.
Alignment is a critical control before cutting. Positioning the head according to a defined anatomical plane helps prevent an uneven division from disrupting the intended correspondence between sides. In Mouse Head Bisecting, the cut is therefore not merely a separation step; it establishes the spatial framework used later for histology, imaging, or molecular analysis.
Bone changes the preparation requirements because skull and dental structures may need to be sectioned or processed with the surrounding soft tissues. When bone must be included, decalcification may be used before subsequent analysis. This option allows the preparation to accommodate mineralized regions while retaining access to the neural, sensory, dental, and facial tissues of interest.
After fixation, the specimen is aligned to the selected anatomical plane and cut through that defined orientation. If bone must be sectioned or processed, decalcification may be incorporated into the preparation. The separated sections can then proceed to histology, imaging, or molecular analysis, with the chosen downstream method matched to the biological question.
Histology reveals tissue organization, imaging provides visual assessment, and molecular analysis extends examination beyond morphology. Because the two sections originate from the same prepared head and preserve spatial relationships, investigators can select the analytical approach that matches the question, whether it concerns structure, pathology, development, or response to treatment.
In biology, the technique is especially relevant when a study must relate several regions of the head rather than examine an isolated tissue. Neural, sensory, dental, and facial structures remain part of the same anatomically organized specimen, allowing investigators to interpret tissue organization and changes within their surrounding spatial context. This supports studies of pathology, development, and treatment effects.