Near Infrared Dyes
Near Infrared Dyes - Related Videos
09:58
An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
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Cited by 14 •
2016
We describe here an optimized protocol of fluorescent Electrophoretic Mobility Shift Assays (fEMSA) using purified SOX-2 proteins together with infrared fluorescent dye-labeled DNA probes as a case study to tackle an important biological question.
10:30
Dye-sensitized Solar Cells
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2023
Source: Tamara M. Powers, Department of Chemistry, Texas A&M University Today's modern world requires the use of a large amount of energy. While we harness energy from fossil fuels such as coal and oil, these sources are nonrenewable and thus the supply is limited. To maintain our global lifestyle, we must extract energy from renewable sources. The most promising renewable source, in terms of abundance, is the sun, which provides us with more than enough solar energy to fully fuel our...
08:11
Infrared Spectroscopy
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2023
Source: Vy M. Dong and Zhiwei Chen, Department of Chemistry, University of California, Irvine, CA This experiment will demonstrate the use of infrared (IR) spectroscopy (also known as vibrational spectroscopy) to elucidate the identity of an unknown compound by identifying the functional group(s) present. IR spectra will be obtained on an IR spectrometer using the attenuated total reflection (ATR) sampling technique with a neat sample of the unknown.
08:36
FM Dyes in Vesicle Recycling
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2023
FM dyes are a class of fluorescent molecules that has found important use in studying the vesicle recycling process. By virtue of a chemical structure, these molecules can insert themselves into the outer leaflet of phospholipid bilayer membranes. After membrane insertion, they are internalized into the cell via endocytosed vesicles, and released when these vesicles recycle back to the membrane. Since, these dyes fluoresce strongly in the hydrophobic environment within membranes and weakly in...
02:35
A Near-Infrared Photoimmunotherapy Technique to Treat Lung Cancer in a Murine Model
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2025
This video demonstrates a near-infrared photoimmunotherapy technique to treat lung cancer in an engineered mouse model exhibiting luciferase-expressing tumor cells. Upon injecting an antibody-photosensitizer conjugate (APC) that binds to specific antigens on the tumor cells, the mouse is exposed to near-infrared radiation, inducing photochemical changes in the APC and leading to necrotic death of the bound tumor cells. The effectiveness of the treatment is monitored by injecting luciferin, a...
FAQs
Conjugated molecular structures shift the dyes’ electronic transitions toward longer wavelengths. When excitation is followed by energy release as near-infrared fluorescence, the emitted signal can be detected from labeled biological targets. This molecular behavior connects chemical structure with imaging performance, allowing researchers to visualize cellular or tissue-associated features.
Near-infrared signals can travel through tissue with relatively low background from endogenous fluorophores, which are naturally present light-emitting molecules. Reduced background can make fluorescence from an attached biological probe easier to distinguish from surrounding tissue. This property is especially relevant to in vivo imaging, where surrounding biological material can otherwise complicate interpretation.
Brightness affects how readily a labeled target produces a detectable signal, while stability helps preserve that signal during an imaging study. Targeting determines whether the dye-bearing probe reaches the intended cells, proteins, blood vessels, or tumors. Optimizing these properties can improve sensitivity and broaden the biological questions addressed by the experiment.
Researchers attach the dye to an antibody, peptide, or another probe chosen for a biological target. The resulting labeled probe can mark cells, proteins, blood vessels, or tumors, depending on the probe’s binding purpose. Imaging then uses the near-infrared signal to observe the selected structure or process in microscopy or in vivo studies.
Their spectral properties support multiplexed detection, meaning an experiment can distinguish signals from more than one labeled target or measurement channel. This capability helps organize information about several biological features within a study rather than restricting observation to a single label. It is useful for microscopy and image-guided investigations that examine complex specimens.
Near-infrared dyes support image-guided studies, diagnostics, and biomedical research by making labeled cells, proteins, blood vessels, or tumors observable. In vivo imaging can connect a fluorescent signal with structures inside living tissue, while microscopy can provide a closer view of labeled biological features. Continued optimization may expand sensitivity and the range of applications.