Nupage System
Nupage System - Related Videos
22:45
Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels
0 Views •
Cited by 28 •
2007
This technical article describes a standard western-blotting procedure using the commercially available NuPAGE electrophoresis Mini-Gel system from Invitrogen.
09:09
Large-Scale Production of Extracellular Vesicles from High-Density 3D Cell Cultures in Hollow Fiber Perfusion Bioreactors
0 Views •
2026
Instructions are provided for setting up and using a 3D perfusion hollow fiber bioreactor for extracellular vesicle production from MSC and other cell types. This protocol describes a method for producing extracellular vesicles at high concentrations on a continuous basis while controlling cell density in hollow fiber bioreactors.
11:08
Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles
0 Views •
Cited by 15 •
2012
Lentiviral expression vectors are the most effective vehicles for stably expressing different effector molecules or reporter constructs in dividing and non-dividing mammalian cells and whole organisms. Here we provide a protocol on how to package lentivector expression constructs in pseudoviral particles and to transduce target cells using the pseudoviral particles.
07:03
In Situ Surface Temperature Measurement in a Conveyor Belt Furnace via Inline Infrared Thermography
0 Views •
Cited by 3 •
2020
This protocol describes how to install an infrared camera into a conveyor belt furnace, conduct a customer correction of a factory calibrated IR camera, and evaluate the spatial surface temperature distribution of an object of interest. The example objects are industrial silicon solar cells.
09:06
The Effect of Construction and Demolition Waste Plastic Fractions on Wood-Polymer Composite Properties
0 Views •
2020
Secondary material streams have been shown to include potential raw materials for production. Presented here is a protocol in which CDW-plastic waste as a raw material is identified, followed by various processing steps (agglomeration, extrusion). As a result, a composite material was produced, and mechanical properties were analyzed.
FAQs
Before electrophoresis, protein samples are typically denatured with sodium dodecyl sulfate and reducing agents. This preparation places the samples in a standardized state for separation through the gel, so differences in the migration pattern can be interpreted primarily in relation to protein mass. The step is therefore central to comparable analysis across samples.
The Bis-Tris gel is paired with a neutral-pH running buffer, commonly MES or MOPS, rather than leaving buffer selection unspecified. This combination defines the electrophoresis environment used by the NuPAGE System. Compared with conventional SDS-PAGE, the precast, standardized format offers a consistent alternative for routine protein separation and comparison.
Migration patterns are read as size-related evidence: proteins that travel to different positions in the Bis-Tris gel can be compared with respect to their molecular mass. This relationship supports molecular-weight estimation and helps reveal whether a sample contains the expected protein pattern. Interpretation should focus on relative migration positions and the overall separation profile.
Because the NuPAGE System uses a standardized precast format, researchers can apply the same general separation platform across experiments. That consistency supports reproducible comparisons of protein migration patterns, expression results, and sample assessments. It is especially useful when biology studies require repeated analyses of related samples rather than a one-time exploratory separation.
A typical workflow begins by preparing protein samples with SDS and reducing agents. The prepared samples are loaded onto a Bis-Tris polyacrylamide gel, and electrophoresis is performed using a neutral-pH MES or MOPS buffer system. Researchers then examine the migration pattern for size-related analysis or use the separated proteins for downstream work.
For protein expression and purity studies, the main readout is the pattern of separated proteins in the gel. Researchers can compare protein migration among samples, estimate molecular weight, and assess whether a preparation shows the expected separation profile. These outcomes make the system useful for checking samples before more specialized protein characterization.
NuPAGE gels can serve as an upstream separation step for Western blotting. After electrophoresis, the resolved protein sample provides an organized basis for subsequent detection by that workflow, while the same separation can also support downstream protein characterization. This makes the system relevant when researchers need both a molecular-size profile and follow-on analysis of selected proteins.