Oral Intubation Mice
Oral Intubation Mice - Related Videos
02:57
Oral Intubation of Adult Zebrafish to Administer Experimental Compounds
0 Views •
2025
This video demonstrates a technique for orally intubating adult zebrafish to administer experimental compounds into the esophagus and monitor their intestinal uptake. This method offers a valuable model for studying the dynamics of oral prophylactics or other compounds within the intestine.
08:46
An Improved Method for Rapid Intubation of the Trachea in Mice
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Cited by 24 •
2016
This article presents a rapid and simple method for administering bleomycin directly into the mouse trachea via intubation. Key advantages of this method are that it is highly reproducible, easy to master, and does not require specialized equipment or lengthy recovery times.
06:58
Endotracheal Intubation in Mice via Direct Laryngoscopy Using an Otoscope
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Cited by 12 •
2014
We have developed a simple, reliable, and relatively inexpensive method for endotracheal intubation in mice via direct laryngoscopy using an otoscope with a 2.0 mm speculum. This technique is atraumatic and can be used for repeated measurements in chronic experiments. We find it superior to tracheostomy or previously reported nonsurgical techniques.
06:26
Repeated Orotracheal Intubation in Mice
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Cited by 2 •
2020
The goal of this article is to describe a refined method of intubation of the laboratory mouse. The method is noninvasive and, therefore, ideal for studies that require serial monitoring of respiratory function and/or instillation of treatments into the lung.
08:38
Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food
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Cited by 28 •
2013
This paper describes a novel method for oral infection of mice using Listeria monocytogenes-contaminated food. The protocol can readily be adapted for use with other food borne bacterial pathogens.
FAQs
Correct tube placement keeps the tracheal passage open, allowing researchers to control airflow during an experiment. This matters because partial or incorrect positioning could compromise ventilation and make exposure less consistent. By stabilizing access to the respiratory tract, the technique supports more comparable measurements across animals and helps investigators interpret pulmonary responses with greater confidence.
Visual guidance helps confirm that the tube is directed toward the trachea rather than misplaced within the airway. It therefore supports accurate positioning while reducing the likelihood of airway injury. This control is especially important when researchers need dependable ventilation, controlled respiratory exposure, or repeatable pulmonary measurements from anesthetized animals.
Anesthesia, careful tube positioning, and consistent handling all influence the quality of the result. Anesthesia supports a controlled procedure, while accurate placement helps maintain airway access without unnecessary injury. Applying the same approach across animals improves experimental consistency, which is essential when comparing ventilation, respiratory treatments, gas exposures, or pulmonary function measurements.
The technique provides a controlled route to the respiratory tract, making the delivery of gases or other experimental treatments more uniform than less controlled exposure approaches. Consistent access also helps standardize ventilation and measurement conditions. As a result, differences in pulmonary function or respiratory responses are more likely to reflect the experimental treatment rather than variation in airway access.
A typical workflow places the mouse under anesthesia, uses visual guidance to identify the airway, and advances a small tube through the mouth into the trachea. Researchers then verify careful positioning before using the access for ventilation or controlled respiratory delivery. Attention to each stage helps maintain a clear airway and minimizes potential injury.
Researchers may select this approach when an experiment requires controlled ventilation, delivery of gases, or administration of treatments directly to the respiratory tract. It is particularly relevant to respiratory biology, pharmacology, toxicology, and physiology studies. The method is useful when consistent exposure and reliable pulmonary measurements are more important than simply observing an unassisted breathing response.
Oral intubation can support measurements of pulmonary function while enabling controlled respiratory exposures and treatments. In respiratory biology, it helps connect airway access with physiological responses; in pharmacology and toxicology, it supports examination of treatment or exposure effects. Reliable tube placement and consistent handling are necessary for those outcomes to be interpreted reproducibly.