Particle-mediated Gene Transfer
Particle-mediated Gene Transfer - Related Videos
03:36
Production of Retroviral Particles for Gene Transfer
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2026
Source: Zjablovskaja, P., et al., Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells. J. Vis. Exp. (2018)This video demonstrates the production of recombinant retroviral particles by transfecting human kidney-derived packaging cells, enabling stable gene delivery to target cells for downstream applications such as genetic modification, functional assays, or lineage differentiation studies.
05:33
Biolistic-Mediated Gene Transfer: A Technique to Deliver Gene of Interest in Target Cells via a Biolistic Gene Gun
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2025
In this video, we demonstrate the protocol of biolistic gene gun-mediated gene delivery in cultured cells. This method provides a simple method of stable genetic transformation using a minimal quantity of DNA.
03:36
Generating Gene Deletions in E. coli via Phage-Mediated Transfer of an Excisable Antibiotic Cassette
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2026
Source: Saragliadis, A., et al. Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes. J. Vis. Exp. (2018)This video demonstrates the phage-mediated transfer of donor DNA carrying an excisable antibiotic resistance cassette into Escherichia coli. The integrated cassette replaces the target gene via homologous recombination, resulting in a stable gene deletion.
16:59
Neo-Islet Formation in Liver of Diabetic Mice by Helper-dependent Adenoviral Vector-Mediated Gene Transfer
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Cited by 5 •
2012
We describe hepatic neo-islet formation in STZ (streptozotocin)-induced diabetic mice by gene transfer of Neurogenin3 (Ngn3) and Betacellulin (Btc) using helper-dependent adenoviral vector (HDAd) and the reversal of hyperglycemia. Our method takes advantages of helper-dependent adenoviral vectors with their highly efficient in vivo transduction and the long lasting gene expression.
03:02
Retroviral Mediated Gene Transduction: A Gene Transfer Technique to Deliver a Transgene in Cultured Cells Using Engineered Retroviral Vectors
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2025
In this video, we demonstrate magnetically-guided transfection of plasmid DNA in primary neuronal cell culture. Magnetofection uses an external magnetic field to guide the delivery of plasmids bound to magnetic nanoparticles into cell cytoplasm.
FAQs
Gold and tungsten particles act as physical carriers for DNA or other genetic material. After the particles are coated with the cargo and accelerated, they can cross cellular barriers that might otherwise limit access to the cell interior. The cargo is then released, allowing genetic material to reach cells or tissues for subsequent gene expression.
The method can support two different expression outcomes: transient expression or stable expression. This flexibility allows investigators to introduce genetic material for experiments focused on gene activity as well as studies seeking a more lasting transformation. The appropriate outcome depends on the biological objective and the cells or tissues receiving the delivered material.
A physical strategy becomes especially useful when biological delivery systems are ineffective or difficult to control. Particle-mediated gene transfer provides a direct way to move genetic material across cellular barriers without relying solely on a biological carrier. This makes it relevant for cells, tissues, or experimental targets that are challenging to address through other delivery approaches.
A basic workflow includes preparing DNA or another genetic payload, coating microscopic gold or tungsten particles with that material, and accelerating the coated particles toward selected cells or tissues. After barrier crossing, the genetic cargo is released and its expression can be evaluated. The approach therefore connects payload preparation, physical delivery, and biological assessment.
Particle-mediated gene transfer supports several research areas, including genetic transformation of plant tissues, cultured cells, and some animal systems. It has also contributed to studies of gene function, crop improvement, and vaccine development. These applications reflect the method’s ability to introduce genetic material into diverse biological targets, including targets that can be difficult to reach.
In biology, the technique links physical access to cells with questions about genetic function and transformation. Researchers can use delivered genetic material to examine gene activity, develop transformed plant material, or investigate delivery strategies relevant to vaccines. Its value extends across plant, cultured-cell, and selected animal systems, broadening experimental options when other methods are limited.