Peripheral Blood Mononuclear Cells
Peripheral Blood Mononuclear Cells - Related Videos
03:14
Ex Vivo Expansion of Natural Killer Cells from Peripheral Blood Mononuclear Cells
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2025
This video demonstrates a technique for the expansion of natural killer (NK) cells directly from peripheral blood mononuclear cells (PBMCs) using a feeder cell line. PBMCs are co-cultured with genetically modified lymphoblastoid feeder cells expressing membrane-bound interleukin-21 (mIL-21), and the culture is supplemented with the cytokines interleukin-2 (IL-2) and interleukin-15 (IL-15). Together, this cocktail of ligands leads to the prolonged expansion of NK cells with high purity.
03:25
Culturing of Activated T Cells from Human Peripheral Blood Mononuclear Cells
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2025
In this video, we describe a protocol for human primary T cell activation. The incubation of naïve T cells with magnetic beads coated with anti-CD3 and anti-CD28 antibodies, followed by interleukin-2 or interleukin-15 treatment, leads to the activation of naïve T cells and their differentiation and proliferation into effector or memory T cells.
02:45
Isolation of Peripheral Blood Mononuclear Cells from Human Buffy Coats
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2025
In this video, we demonstrate the isolation of peripheral blood mononuclear cells, PBMCs, containing monocytes and lymphocytes from a human buffy coat by density gradient centrifugation. The isolated PBMCs can be used for further analysis.
09:45
Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
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Cited by 17 •
2017
This protocol describes a detailed method for efficient generation of integration-free iPSCs from human adult peripheral blood cells. With the use of four oriP/EBNA-based episomal vectors to express the reprogramming factors, KLF4, MYC, BCL-XL, or OCT4 and SOX2, thousands of iPSC colonies can be obtained from 1 mL of peripheral blood.
03:06
Negative Immunomagnetic Selection: A Method to Purify B-cells from Peripheral Blood Mononuclear Cells
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2023
The video explains a way to purify B-cells by immunomagnetic targeting and separation from peripheral blood mononuclear cells suspension. The method involve labeling unwanted cell types with specific antibodies or ligands (linked to magnetic particles) targeting specific cell surface proteins, to be depleted from the heterogenous cell suspension by magnetic separation.
FAQs
The method separates blood components according to differences in cellular density. During centrifugation, mononuclear cells are recovered apart from denser erythrocytes and granulocytes, allowing investigators to enrich the fraction containing lymphocytes and monocytes. This physical separation is important because it produces a cell population suitable for downstream immune analysis rather than examining all whole-blood components together.
Lymphocytes and monocytes represent major immune cell populations within the PBMC fraction, so their responses can reveal changes in immune surveillance and activation. Examining these cells helps researchers assess how blood-based immunity reacts to infections, inflammatory conditions, vaccines, drugs, or antigens. Their combined presence also permits analysis of coordinated responses across more than one immune-cell type.
PBMC analysis focuses on cells with a single, round nucleus, whereas erythrocytes and granulocytes are separated during preparation because their cellular densities differ. This distinction directs experiments toward immune-cell behavior rather than the properties of the other blood-cell fractions. Consequently, PBMC-based assays are particularly suited to studying immune activation, cellular responses, and disease-related immune changes.
The information depends strongly on the analytical approach applied after isolation. Flow cytometry can characterize cellular responses, culture can examine behavior under experimental conditions, and molecular assays can investigate associated molecular changes. The biological question also matters: infection, inflammation, vaccination, hematologic disorders, drugs, and antigens each provide a different context for interpreting PBMC responses.
A typical workflow begins with collecting whole blood from a living donor, followed by density-gradient centrifugation to separate the mononuclear fraction from erythrocytes and granulocytes. The recovered PBMC population can then be directed to culture, flow cytometry, or molecular assays. This sequence connects practical cell collection with experiments that evaluate human immune function or disease biology.
PBMCs are useful when investigators need a living-donor source of immune cells that can be examined outside the original blood sample. In infection and inflammation research, they support assessment of immune activation and related cellular responses. In vaccination studies, researchers can analyze how immune cells respond to the vaccine context, while antigen-based experiments provide another way to probe immune reactivity.
Isolated PBMCs can be exposed to drugs or antigens and then evaluated with culture, flow cytometry, or molecular assays. These approaches allow researchers to examine cellular responses rather than relying only on whole-blood observations. The resulting measurements can help investigate immune activity, compare response patterns, and study how candidate treatments or antigens affect human immune cells.
PBMCs provide access to blood-derived immune cells from living donors, creating a practical system for examining biology associated with hematologic disorders. Researchers can combine cell culture, flow cytometry, and molecular assays to characterize immune-cell behavior and related changes. This makes the preparation useful for connecting cellular observations with broader questions about disease biology and human immune function.