Peritoneal Incision
Peritoneal Incision - Related Videos
04:32
Isolation of Mouse Peritoneal Cavity Cells
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Cited by 445 •
2010
The peritoneal cavity in mammals contains different immune cell populations crucial for innate immune responses. An efficient isolation method is required for biochemical and functional analyses of these cells. Here we provide a comprehensive method for the isolation of peritoneal cavity cells in the mouse.
03:41
Harvesting Peritoneal Fat Depots: A Technique to Study Ovarian Cancer Colonization of Peritoneal Adipose Tissues in Mouse Models
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2023
In this video, we use a euthanized female mouse model bearing ovarian cancer. We then describe a protocol to identify and extract fat depots or adipose tissues associated with the five primary peritoneal organs to study metastatic cancer cell colonization.
05:05
In Vivo Activation of Peritoneal Macrophages in Response to Antibodies in a Murine Model
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2025
This video demonstrates the activation of a mouse's peritoneal macrophages using intravenous immunoglobulin and bacterial lipopolysaccharide. The co-stimulation activates the peritoneal macrophages into anti-inflammatory macrophages possessing immune regulatory activity.
05:36
Analysis of Efferocytosis of Apoptotic Thymocytes by Peritoneal Macrophages
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2025
This video demonstrates a procedure for analyzing peritoneal macrophage-mediated efferocytosis of apoptotic mouse thymocytes by fluorescence microscopy.
04:17
A Technique to Isolate Primary Mast Cells from a Murine Peritoneal Cavity
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2025
This video demonstrates a peritoneal lavage method for isolating mature connective tissue-type mast cells from the peritoneal cavity of adult mice. Upon isolating a mixture of primary immune cells from the peritoneal cavity, selective proliferation of mast cells is performed under appropriate culture conditions.
FAQs
Controlled access depends on opening the peritoneal tissue while limiting injury to nearby structures. This balance allows surgeons or researchers to reach abdominal organs or selected tissues without creating unnecessary disruption. The quality of that access matters because the extent of peritoneal disturbance can influence subsequent healing, inflammation, adhesion formation, and restoration of the abdominal barrier.
Limiting damage helps distinguish the intended surgical or experimental effect from additional tissue responses caused by unnecessary disruption. A carefully performed opening can support access while reducing factors that may complicate recovery or alter observations of inflammation, adhesions, and tissue repair. This is especially important in studies that evaluate how the peritoneum responds after injury.
Closure returns the disrupted membrane toward continuity and allows healing to restore the abdominal barrier. It also marks the transition from creating access to monitoring tissue repair. In biomedical research, the post-closure response can provide information about healing quality, inflammation, adhesion development, and the broader effects of repairing a surgically disturbed peritoneal surface.
Disruption of the peritoneum can affect recovery and provoke tissue responses that are relevant to both clinical surgery and biology research. Investigators may examine inflammation, adhesion formation, or repair as outcomes associated with the incision and subsequent closure. These responses help characterize how tissues react when a normally continuous abdominal membrane is deliberately disturbed and then repaired.
The essential sequence is to create a controlled opening, maintain access while limiting damage to nearby structures, and close the incision afterward. Healing then allows the membrane to restore the abdominal barrier. The same broad sequence supports clinical abdominal operations and experimental work, although the specific purpose may differ between access, sampling, implantation, or tissue-response studies.
Applications include abdominal surgery, tissue sampling, implantation procedures, and experimental studies of wound healing, inflammation, adhesions, and tissue repair. Its value extends beyond access alone: the incision provides a defined tissue disruption whose healing and postoperative responses can be examined. This makes it relevant for connecting surgical technique with biological mechanisms of recovery.