Radioisotope Labeling
Radioisotope Labeling - Related Videos
01:28
Isotopes and Radioisotopes
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2023
In the early 1900s, English chemist Frederick Soddy realized that an element could have atoms with different masses that were chemically indistinguishable. These different types are called isotopes — atoms of the same element that differ in mass. Isotopes differ in mass because they have different numbers of neutrons but are chemically identical because they have the same number of protons. Soddy was awarded the Nobel Prize in Chemistry in 1921 for this discovery. An isotope containing more...
12:48
Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays
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Cited by 7 •
2013
We describe a method to quantify the activity of K+-countertransporting P-type ATPases by heterologous expression of the enzymes in Xenopus oocytes and measuring Rb+ or Li+ uptake into individual cells by atomic absorption spectrophotometry. The method is a sensitive and safe alternative to radioisotope flux experiments facilitating complex kinetic studies.
07:23
An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
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Cited by 5 •
2016
A detailed procedure for the synthesis of a 125I-labeled azide and the radiolabeling of dibenzocyclooctyne (DBCO)-group-conjugated, 13-nm-sized gold nanoparticles using a copper-free click reaction is described.
09:19
Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
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Cited by 3 •
2025
This protocol presents a step-by-step guide for the Simultaneous Label-free Autofluorescence Multi-harmonic (SLAM) microscopic technique, including details on how to generate the laser light source, prepare a tissue sample, conduct imaging, and analyze the data. SLAM advances nonlinear microscopy by measuring four complementary label-free contrasts to investigate the tissue microenvironment.
08:28
Metabolic Labeling
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2023
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
FAQs
A radioisotope is incorporated into a compound while leaving its chemical properties substantially unchanged. This allows the labeled molecule to participate in biological processes in a representative manner, so measurements can follow its movement or transformation. Preserving molecular behavior is important when interpreting transport, metabolism, nucleic acid synthesis, or biomolecular interactions.
Radioactive emissions provide a sensitive signal that can be measured as labeled molecules change location or abundance over time. Methods such as autoradiography, scintillation counting, and radiation detection convert that signal into quantitative information. This sensitivity helps researchers examine biological events that are not readily observed through conventional chemical analysis alone.
Repeated measurements of radioactive emissions show how the amount or distribution of a labeled compound changes with time. Those changes can be used to examine reaction kinetics, including the progression of a biological process. The time-dependent signal also helps connect molecular behavior with broader events such as transport or metabolic conversion.
A typical workflow begins by incorporating the radioisotope into the compound of interest and allowing the labeled material to participate in a biological system. Researchers then collect radioactive measurements over time using an appropriate detection method. The resulting signal is analyzed to determine molecular transport, pathway behavior, synthesis, interactions, or reaction progress.
Three measurement approaches identified for this method are autoradiography, scintillation counting, and radiation detection. Autoradiography can show where radioactivity is associated with a sample, whereas counting and detection approaches provide measured signal values. Selecting among them depends on whether the study emphasizes location, amount, or changes in radioactivity over time.
The approach is useful when researchers need quantitative evidence about molecular movement or activity inside biological systems. Applications include examining cell function, tracing metabolic pathways, following nucleic acid synthesis, assessing drug distribution, and studying biomolecular interactions. It is especially valuable when the relevant process cannot be clarified adequately through ordinary chemical measurements.