Rna Extraction
Rna Extraction - Related Videos
05:38
RNA Extraction Assay: A Method to Extract RNA from miRNA Transfected Lung Cancer Cells
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2023
This video describes the RNA extraction protocol from miRNA transfected lung cancer cells. This approach can be used to identify a combinatorial miRNA treatment activity in halting the cell cycle and angiogenesis.
17:25
RNA Extraction from Neuroprecursor Cells Using the Bio-Rad Total RNA Kit
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Cited by 1 •
2007
05:07
RNA-Protein Pull-Down Assay to Isolate RNA-Binding Proteins via Affinity Extraction
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2025
This video demonstrates an in vitro RNA pull-down assay to identify RNA-binding proteins (RBPs), which interact with the adenylate-uridylate-rich element (ARE) sequences in mRNA. The target RBPs from a cell lysate are mixed with an RNA probe to form RNA-protein complexes. The complexes are isolated via affinity purification utilizing the affinity of the desthiobiotin label of the RNA probe to a streptavidin-labeled magnetic bead.
04:33
Circulating miRNA Extraction: A Method to Isolate miRNA from Plasma Samples Via Organic Extraction and Small RNA Enrichment
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2023
This video describes the extraction of circulating microRNA from plasma samples of colorectal cancer patients using organic extraction followed by glass-fiber column enrichment. miRNAs are stable in biological fluids such as serum and plasma, which renders them ideal circulating biomarkers for cancer diagnosis, prognosis, and treatment decision-making and monitoring. This method will help identify new circulating biomarkers capable of predicting clinical outcomes in patients.
02:59
Extraction of Viral RNA from Surface-Bound Virions Using a Silica Spin Column
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2026
Source: Klaus, J. P. et al. Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment. J. Vis. Exp. (2016)This video demonstrates the extraction of viral RNA from surface-bound virions on epithelial cells using silica spin columns. The method includes chemical lysis, RNA stabilization, and membrane-based purification for downstream quantification of virus-cell attachment.
FAQs
RNases can degrade RNA during sample handling, reducing the amount and integrity of the material available for analysis. RNA extraction therefore includes conditions that inactivate these enzymes, along with careful handling and preservation. Protecting the molecules from degradation helps maintain a more reliable molecular snapshot of cellular or biological activity for downstream measurements.
The workflow separates RNA from cellular components so the recovered material is enriched for the nucleic acid of interest. Removing DNA, proteins, and other molecules reduces unwanted material that could interfere with later analyses. This purification step is particularly important when the RNA will support reverse transcription, quantitative PCR, sequencing, or gene-expression studies.
RNA integrity affects how faithfully an extracted sample represents the biological state from which it came. Degraded or poorly preserved RNA may provide a less reliable molecular snapshot, limiting interpretation of gene activity or cellular responses. For this reason, preservation and contamination control are not merely technical details; they directly support trustworthy biological conclusions.
A typical workflow begins by disrupting cells, tissues, or another biological sample, followed by inactivating RNases that could damage the RNA. The process then separates RNA from DNA, proteins, and other molecules before purification and concentration. Maintaining contamination control and preserving the material throughout these stages supports a usable preparation for subsequent analysis.
Recovered RNA can be used in several downstream approaches, including reverse transcription, quantitative PCR, RNA sequencing, and gene-expression analysis. These methods allow researchers to examine RNA-related patterns rather than treating extraction as the final objective. The selected analysis determines what information can be obtained from the purified material, such as gene activity or biological response.
In biology, extracted RNA helps investigate molecular activity across genetics, development, disease, and biotechnology. It can also support studies of viral responses and cellular responses, linking recovered RNA to changes occurring in biological systems. Because the material reflects activity at the time of sampling, extraction connects a biological specimen with later molecular analysis.