Simple Transposition
Simple Transposition - Related Videos
02:13
Overview of Transposition and Recombination
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2020
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
04:42
Generating a Rat Model of Tibial Neuroma Transposition
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2025
Source: Brakkee, E. M., et al. Surgery and Behavioral Testing in the Tibial Neuroma Transposition Model in Rats. J. Vis. Exp. (2023).This video demonstrates a procedure to generate a rat tibial neuroma transposition (TNT) model. After exposing the tibial nerve in the hind leg of an anesthetized rat, transect the nerve. Next, create a subcutaneous tunnel, thread the severed nerve through the tunnel, and suture it in place. In the absence of a target for reinnervation, disorganized axonal...
03:13
Simple Distillation - Concepts
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2020
Distillation of a Miscible Mixture Some liquids will evaporate entirely over time if left in an open container at room temperature. However, this evaporation process can be significantly accelerated if the liquid is heated. As the liquid is heated, the molecules within it gain the energy to escape the liquid phase and transition into the gas phase in the form of bubbles. This phenomenon is called boiling. Vapor Pressure Consider a closed container of liquid. Initially, some of this liquid...
06:49
Simple Distillation - Student Protocol
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2020
Source: Lara Al Hariri and Ahmed Basabrain at the University of Massachusetts Amherst, MA, USA Simple DistillationExpand In this lab, you will use simple distillation to separate a mixture of cyclohexane and toluene. Before you start the lab, put on the appropriate personal protective equipment, including a lab coat, safety goggles, and gloves. This experiment must be conducted in a hood. Assemble the distillation apparatus. Refer to the example set up that has been prepared...
05:11
Glioblastoma Induction Using SB Mediated Transposition: A Technique for Generating Novel Mouse Model Using Transposon Mediated Integration of SB Plasmid into Neonatal Mouse Brain
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2025
This video describes an efficient technique to induce glioblastomas (GBM) using transposon DNA injected into the ventricles of neonatal mice. Cells of the subventricular zone, which take up the plasmid, transform, proliferate, and generate tumors with histopathological characteristics of human GBM.
FAQs
Terminal sequences act as recognition signals for the transposase, the enzyme that manages the element’s movement. Their recognition enables the enzyme to identify the mobile DNA, remove it from the donor location, and support insertion at another genomic target. This sequence-specific recognition is therefore central to controlling which DNA segment participates in the rearrangement.
Insertion can produce short duplicated DNA sequences flanking the newly inserted element. These duplications mark the target-site changes associated with the rearrangement and provide evidence that insertion altered the local genome sequence. Examining such flanking sequences helps researchers identify where a mobile element entered and distinguish the insertion event from other genome changes.
A newly inserted element can interrupt a gene directly or alter a nearby regulatory region that influences gene activity. Either outcome may change cellular traits by modifying genetic information or its control. Because the element can relocate to different genomic positions, repeated events may generate mutations and broader genome rearrangements that contribute to genetic variation.
In insertional mutagenesis, researchers use movement of a transposable element to introduce a new DNA insertion into the genome. If the insertion disrupts a gene or regulatory region, the resulting mutation can reveal that region’s contribution to a biological function. This makes transposition-based approaches useful for creating genetic changes that can be examined experimentally.
A transposable element can serve as a way to alter genomic regions and then associate the resulting genetic change with an observed biological effect. When an insertion disrupts a gene, comparing the altered system with an unaltered one can support gene-function analysis. The approach is especially informative when the insertion location and affected region can be identified.
Movement of mobile genetic elements changes genome structure by relocating DNA, disrupting genes, modifying regulatory regions, and producing mutations or rearrangements. Over time, these changes can contribute to differences among genomes and provide material for studying genome evolution. In biology, transposition therefore connects molecular DNA rearrangement with larger patterns of genomic diversity.