Time-restricted Feeding

Time-restricted feeding (TRF) is a dietary pattern that limits food intake to a consistent daily window while extending the period without calories, making it a useful model for studying interactions among nutrition, metabolism, and biological timing. During the fasting interval, reduced nutrient availability shifts cellular metabolism away from repeated feeding signals, while the timing of meals can interact with circadian rhythms that coordinate metabolic processes across tissues. In biology, TRF is used to investigate energy balance, glucose regulation, sleep-wake cycles, and the effects of meal timing on health and disease. Research using this approach may clarify how behavioral schedules influence physiology and inform nutrition, aging, and metabolic disease studies.

Time-restricted Feeding - Related Videos

Education

JoVE Science Education - Basic Biology

Restriction Enzyme Digests

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2023

Restriction enzymes or endonucleases recognize and cut DNA at a specific sequence. These enzymes occur naturally in bacteria as a defense against bacteriophages - viruses that infect bacteria. Bacterial restriction enzymes cut the invading bacteriophage DNA while leaving the bacterial genomic DNA unharmed due to addition of methyl groups. This video explains the basic principles of restriction enzymes including: how restriction enzymes are named and the types of recognition sites and...

Research

JoVE EoE - Drosophila melanogaster (fruit fly)

Feeding Test: Assessment of Feeding Preferences in Drosophila melanogaster

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2023

This video describes a feeding assay for fruit flies in which colored food is used to monitor feeding and the effect added compounds may have on feeding activity. The example protocol tests the effects of several endocrine disrupting chemicals (EDC).

Research

JoVE Journal - Biology
Free Sample

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

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Cited by 9 •

2017

This protocol outlines a simple method for analyzing calcium signals in plants generated by feeding hemipteran insects, such as aphids. Arabidopsis thaliana transformed with the GFP calcium biosensor GCaMP3 allow for the real-time in vivo imaging of calcium dynamics with a high temporal and spatial resolution.

Education

JoVE Lab Manual - Biology
Free Sample

DNA Isolation and Restriction Enzyme Analysis - Student Protocol

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2019

DNA Isolation ExpandNOTE: In this experiment you will perform DNA isolation under two experimental conditions: one using a buffer containing the detergent SDS and one without detergent. Hypotheses: The alternate hypothesis for this experiment might be that the sample prepared without SDS, a strong anionic detergent that breaks apart cell membranes, will yield less DNA than the sample prepared with SDS. The null hypothesis for this experiment might be that both samples will yield an equal amount...

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

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Cited by 19 •

2011

Here we present a protocol for performing solid plate-based dietary restriction in C. elegans with killed bacteria.

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