Tissue Culture Insert
Tissue Culture Insert - Related Videos
11:56
Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
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Cited by 1 •
2010
Fabrication and validation of an add-on platform that offers enhanced control over the spatial and temporal oxygenation in a 6-well plate. The device is adaptable to a number of culture systems and can be used to investigate the effects of oxygen on wound healing.
11:29
Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact
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Cited by 31 •
2016
In multicellular organisms, secreted soluble factors elicit responses from different cell types as a result of paracrine signaling. Insert co-culture systems offer a simple way to assess the changes mediated by secreted soluble factors in the absence of cell-cell contact.
08:45
Whole Organ Tissue Culture
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2023
Whole organs can be cultured ex vivo using specialized bioreactors with the goal of repairing or replacing entire organs. This method uses a donor organ that is stripped of all cells, leaving behind the three-dimensional structure, which is then repopulated with new cells. This video demonstrates the whole organ culture of lungs and shows how a dynamic culture that mimics the mechanical stimulation in the body is needed to induce native tissue properties.
09:35
Histotypic Tissue Culture
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2023
Although two-dimensional tissue culture has been common for some time, cells behave more realistically in a three-dimensional culture, and more closely mimics native tissue. This video introduces histotypic tissue culture, where the growth and propagation of one cell line is done in an engineered three-dimensional matrix to reach high cell density. Here, we show the harvesting of cells from donor tissue, followed by cell culture on an engineered construct.
06:17
Nanofibrillar Basement Membrane Mimic Made of Recombinant Functionalized Spider Silk in Custom-Made Tissue Culture Inserts
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Cited by 1 •
2024
Tissue culture inserts with plastic membranes are the golden standard in cell culture labs as permeable supports to establish cell layers and models of barrier tissues. Herein, we present a simple method to replace the plastic membrane with a more biologically relevant membrane made from a recombinant functionalized spider silk protein.
FAQs
The porous membrane creates separate apical and basolateral environments without completely isolating the cells from one another. Nutrients, signaling molecules, and experimental compounds can diffuse across the interface, while researchers maintain or sample each medium independently. This arrangement helps distinguish effects arising at the cell surface from changes detected in the opposing compartment.
Polarized cells organize different functions toward opposite sides, so exposing each surface to a distinct medium better reflects tissue organization than a single undivided culture environment. A tissue culture insert allows researchers to examine directional transport, barrier behavior, and responses to compounds or pathogens according to the compartment they contact.
The interface supports analysis of epithelial permeability, transport, cell migration, and communication between cellular populations. Researchers can also assess how cells respond to drugs or pathogens while tracking changes in separate compartments. These measurements connect membrane passage and cellular behavior with the function of a developing biological barrier or tissue model.
A typical workflow establishes cells on the insert membrane, maintains distinct media on the two sides, and allows the cellular layer or barrier to develop before testing a condition. Researchers may then introduce an experimental compound or pathogen, monitor the response, and collect samples separately from the apical and basolateral compartments.
They are useful when researchers need two cellular populations to communicate through soluble signals while remaining in separate compartments. The membrane maintains physical separation but permits diffusion across the interface, making it possible to examine co-culture interactions and determine how one population influences the behavior or barrier properties of another.
Tissue culture inserts support models of intestinal, respiratory, and vascular tissues, where compartmental organization and barrier function are important. Within these systems, researchers can investigate permeability, transport, cellular communication, and tissue responses to drugs or pathogens. Sampling both sides helps relate localized exposure to changes elsewhere in the model.