Transmission Light
Transmission Light - Related Videos
05:33
Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction
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Cited by 2 •
2022
The present protocol describes transmission electron microscope (TEM) modifications with a light illumination system, the fabrication of liquid cells, and in situ TEM observations of light-induced interactions between bacterial cells and a photosensitizer. The sample preparation methods, electron beam damage, and imaging are also discussed.
02:12
The Wave Nature of Light
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2020
The nature of light has been a subject of inquiry since antiquity. In the seventeenth century, Isaac Newton performed experiments with lenses and prisms and was able to demonstrate that white light consists of the individual colors of the rainbow combined together. Newton explained his optics findings in terms of a "corpuscular" view of light, in which light was composed of streams of extremely tiny particles traveling at high speeds according to Newton's laws of motion. Others in the...
09:43
Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping
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Cited by 10 •
2017
We demonstrate the transmission of multiple independent signals through a multimode fiber using wavefront shaping employing a single spatial light modulator. By modulating the wavefront for each signal individually, spatially separated foci are transmitted. Potential applications are multiplexed data transfer in communications engineering and endoscopic light delivery in biophotonics.
09:30
Non-invasive Assessment of Changes in Corticomotoneuronal Transmission in Humans
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Cited by 6 •
2017
The aim of the present study was to assess changes in transmission at the corticomotoneuronal synapses in humans after repetitive transcranial magnetic stimulation. For this purpose, an electrophysiological method is introduced that allows assessment of pathway specific corticospinal transmission, i.e. differentiation of fast, direct corticospinal pathways from polysynaptic connections.
02:53
Ultrastructural Analysis of a Mouse Brain Section Using Transmission Electron Microscopy
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2025
Source: Lutz, D., et.al. Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord. J. Vis. Exp. (2019).This video demonstrates the preparation and transmission electron microscopy (TEM) analysis of a brain section from a mouse pup. The protocol starts with osmium tetroxide-fixed tissue embedded in resin, followed by precise trimming to isolate the region of interest and ultramicrotomy to produce semithin and ultrathin...
FAQs
Image contrast arises when different parts of a specimen alter the transmitted illumination to different degrees. Absorption reduces the light reaching the instrument, while scattering redirects it, and refractive-index differences change how light travels through materials. These variations convert otherwise optically similar regions into distinguishable patterns, supporting analysis of cellular or tissue morphology.
Stains and phase-contrast methods enhance features that produce weak contrast under ordinary illumination. Staining can make selected structures more visually distinct, whereas phase contrast helps reveal differences that may otherwise be difficult to separate in the image. These enhancements improve the visibility of internal organization and support more reliable morphological observation.
The approach works best when light can pass through the specimen sufficiently for internal features to influence the image. Thin or transparent biological material allows optical instruments to capture differences within the sample rather than only its surface. This makes the method useful for examining cells, tissues, microorganisms, and developmental structures while retaining their spatial relationships.
A basic workflow begins by positioning a thin or transparent specimen between the illumination source and the optical instrument. The transmitted image is then examined for contrast produced by absorption, scattering, or refractive-index differences. If structures remain difficult to distinguish, staining or phase-contrast methods can enhance visibility, enabling morphological or quantitative analysis.
Transmission light imaging is useful for routine morphology, histology, cell biology, and studies of development. It can reveal cells, tissues, microorganisms, and developmental structures in a shared spatial context. Researchers can therefore examine organization and form across biological specimens, using the same general optical strategy for descriptive observation or quantitative study of structure and growth.
Images can preserve relationships among internal structures, allowing investigators to assess organization rather than viewing isolated features alone. The same data can support quantitative studies of structure and growth, in addition to routine visual inspection. Interpretation depends on the contrast generated in the specimen and on whether stains or phase contrast have made relevant features sufficiently distinguishable.