Transpiration Pull

Transpiration pull is the force that moves water upward through a plant’s xylem as water evaporates from leaves, making it essential for plant hydration and mineral transport. When stomata release water vapor, evaporation creates negative pressure, or tension, in the leaf; cohesion between water molecules and adhesion to xylem walls transmit this tension through a continuous water column from roots to shoots. This cohesion-tension mechanism links leaf gas exchange with long-distance transport and helps explain how tall plants move water without a mechanical pump. Understanding transpiration pull supports studies of plant physiology, drought responses, irrigation, and how environmental conditions influence growth and productivity.

Transpiration Pull - Related Videos

Education

JoVE Lab Manual - Biology

Transpiration - Concepts

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2019

Background All living organisms must carry out a set of basic functions in order to maintain themselves. One of these processes involves the transportation of materials throughout the organism. Therefore, organisms need to exchange materials with the environment, which can be seen on the small scale of cells transporting protein and other materials among one another or on a larger scale such as the water cycle, where water is continuously moving on, above, and below the surface of Earth. In...

Transpiration - Student Protocol

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2019

Comparing Transpiration Rates of Leaves ExpandTo begin the experiment to measure the transpiration rates of leaves from different plant species, you will first create a tool called a potometer, using a piece of plastic tubing and 5 mL calibrated pipette. To assemble the device, simply attach the rubber tubing to the tip of the pipette. Then, submerge the device into a bucket of water and move the pipette around under the water to fully fill the tube, taking care not to create bubbles. Next, cap...

Transpiration - Instructor Prep

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2019

Comparing Transpiration Rates of Leaves ExpandBefore beginning the experiments, gather leaves from four plant species that represent different ecological strategies, so that you have enough for one set of leaves per student. NOTE: If leaf-bearing plants are not available locally, an alternative is to grow the plant species of interest in the laboratory, or to purchase plants from a gardening store. Next, cut lengths of rubber tubing to make roughly 50 cm sections and place one piece alongside a...

Research

JoVE Journal - Biology

Pull-down of Calmodulin-binding Proteins

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Cited by 19 •

2012

Calmodulin (CaM) pull-down assay is an effective way to investigate the interaction of CaM with various proteins. This method uses CaM-sepharose beads for efficient and specific analysis of CaM-binding proteins. This provides an important tool to explore CaM signaling in cellular function.

Regulation of Transpiration by Stomata

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2026

During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.Each stoma is flanked by two specialized guard cells that create an opening when these cells take up water. The transport of ions regulates the...

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