Vascular Network Imaging
Vascular Network Imaging - Related Videos
10:53
Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
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Cited by 10 •
2017
This protocol outlines the implementation of image-guided, laser-based hydrogel degradation to fabricate vascular-derived, biomimetic microfluidic networks embedded in poly(ethylene glycol) diacrylate (PEGDA) hydrogels. These biomimetic microfluidic systems may be useful for tissue engineering applications, generation of in vitro disease models, and fabrication of advanced "on-a-chip" devices.
03:33
Visualization of Neural and Vascular Networks in a Chicken Embryo
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2025
Source: Delalande, J., et.al. Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection. J. Vis. Exp. (2015)This video demonstrates the transplantation of a GFP-labeled donor neural tube from a stage-matched transgenic chicken embryo into a recipient embryo at the level of somites one to seven, followed by vascular labeling using a lipophilic fluorescent dye. The combined approach allows for direct...
16:33
Functional Calcium Imaging in Developing Cortical Networks
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Cited by 32 •
2011
Spontaneous activity of developing neuronal networks can be measured using AM-ester forms of calcium-sensitive indicator dyes. Changes in intracellular calcium, indicating neuronal activation, are detected as transient changes in indicator fluorescence with one- or two-photon imaging. This protocol can be adapted for a range of developmentally-dependent neuronal networks in vitro.
06:54
An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks
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Cited by 18 •
2017
Angiogenesis involves multi-cell, multi-system interactions that need to be investigated in a physiologically relevant environment. The objective of this study is to demonstrate the ability of the rat mesentery culture model to make time-lapse comparisons of intact microvascular networks during angiogenesis.
08:05
Doppler Ultrasonography for Live Imaging and Quantification of Ovarian Vascular Function in Mice
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Cited by 1 •
2025
Changes in ovarian blood flow during the preovulatory period are essential for ovulation. Doppler ultrasonography has been used in humans and large animals to assess ovarian hemodynamics and perfusion. A protocol is presented for applying Doppler ultrasonography to evaluate ovarian hemodynamics during the preovulatory and periovulatory phases in mice.
FAQs
Measurements of vessel density, diameter, branching patterns, and connectivity provide complementary indicators of network organization. Comparing these features between tissues or biological conditions helps researchers characterize angiogenesis, vascular development, and remodeling rather than relying on a single structural measurement. The resulting profile can show whether changes involve vessel abundance, geometry, or network continuity.
Image contrast is central to separating vessels from neighboring tissue. Fluorescence labeling or contrast enhancement marks the vascular structures, while microscopy records the resulting signal or contrast. Three-dimensional image reconstruction can then preserve spatial relationships that may be difficult to interpret in isolated views, supporting analysis of vessel organization and connectivity within the tissue.
Branching patterns describe how vessels are organized, whereas connectivity addresses how different portions of the network relate to one another. Examining both measurements gives a more complete view of vascular architecture than either feature alone. This combined information helps link structural organization with tissue perfusion and cellular function in biological studies.
A basic workflow begins by making vessels distinguishable through fluorescence labeling or contrast enhancement. Microscopy is then used to capture the vascular features, and three-dimensional reconstruction may organize those observations spatially. Researchers can quantify vessel density, diameter, branching, and connectivity afterward, producing measurements suitable for comparing vascular networks across tissues, organisms, or experimental conditions.
The approach is useful when vascular structure must be related to a biological process or tissue outcome. Applications described for this method include studies of angiogenesis, vascular development, tissue perfusion, disease-associated remodeling, tumor biology, wound healing, and engineered tissues. In each setting, structural measurements provide a way to examine how vascular organization changes with biological context.
Quantified vascular features create a structural basis for interpreting biological effects. Vessel density, diameter, branching, and connectivity can be considered alongside cellular function or physiological outcomes to examine how network organization relates to tissue behavior. This perspective is especially relevant in tumor biology, wound healing, and engineered tissues, where vascular remodeling may accompany changes in tissue state.