Whitefly Dissection
Whitefly Dissection - Related Videos
10:22
Transmitting Plant Viruses Using Whiteflies
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Cited by 41 •
2013
Improving our understanding of and our ability to manage many of the insect-transmitted plant viruses requires the use of the vector. Insect transmission of plant viruses is a tritrophic interaction, and as such requires the manipulation of insects, virus, and plant. Vectors must be reared in large numbers and manipulated in such a way as to insure high rates of transmission to test plants. The basics of rearing and manipulating the whitefly, Bemisa tabaci (Hemiptera: Aleyrodidae), a vector of...
07:28
Methods for the Extraction of Endosymbionts from the Whitefly Bemisia tabaci
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Cited by 4 •
2017
Here, we present a protocol to isolate endosymbionts from the whitefly Bemisia tabaci through dissection and filtration. After amplification, the DNA samples are suitable for subsequent sequencing and study of the mutualism between endosymbionts and whitefly.
06:25
Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR
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Cited by 5 •
2020
We describe an immunofluorescence and quantitative PCR method for the localization and quantification of begomoviruses in insect tissues. The immunofluorescence protocol can be used to colocalize viral and vector proteins. The quantitative PCR protocol can be extended to quantify viruses in whole whitefly bodies and virus-infected plants.
10:18
Dissection of Organs from the Adult Zebrafish
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Cited by 167 •
2010
This protocol describes a procedure for identifying and dissecting organs from the adult zebrafish.
07:40
Dissection of the Adult Zebrafish Kidney
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Cited by 38 •
2011
The zebrafish kidney is home to both renal and hematopoietic adult stem/progenitor cells, and represents an outstanding opportunity to study these cell types and their progeny in a vertebrate model organism. Here, we demonstrate a detailed dissection procedure that enables the researcher to identify and surgically remove the adult zebrafish kidney, which can be used for applications such as cell isolation, transplantation, and expression studies of kidney and/or blood cell populations.
FAQs
The process combines immobilization, cuticle opening, and tissue separation under a stereomicroscope. Immobilizing the insect keeps the specimen positioned while fine tools open the outer covering and allow researchers to distinguish internal tissues. This sequence makes it possible to examine digestive and reproductive systems alongside external anatomy rather than viewing body structures in isolation.
Fine tools provide the control needed to open the cuticle and separate tissues without obscuring the structures being studied. Tissue separation is especially important when researchers need to distinguish the digestive system from reproductive structures. Careful handling improves anatomical observation and supports more reliable comparisons of whitefly morphology under the microscope.
Observations of the digestive and reproductive systems provide anatomical context for studying feeding behavior and reproduction. Examining these structures helps researchers connect body organization with how whiteflies obtain plant resources and produce offspring. This structure-function perspective is useful in biology because it links microscopic anatomy with physiological processes and the insect’s effects on plants.
Detailed views of external and internal anatomy can support comparisons among whiteflies and across developmental contexts. Such comparisons contribute to morphology-based taxonomy, which uses structural features to study classification, while also helping researchers investigate how anatomy changes or differs during development. The technique therefore supplies biological observations that complement broader studies of whitefly identity and life history.
A basic workflow begins by immobilizing the whitefly for examination under a stereomicroscope. Researchers then use fine tools to open the cuticle and separate tissues, making internal structures available for observation. The resulting preparation can be examined for external features as well as digestive and reproductive anatomy, depending on the biological question being investigated.
The technique is useful when researchers need anatomical evidence related to whitefly morphology, development, physiology, or taxonomy. In plant biology and agriculture, those observations can also support studies of feeding and the insects’ effects on crop health. Anatomical examination provides a way to connect organismal structure with questions about whitefly biology and plant-pathogen interactions.