Hydrophobic Barrier Pen
Hydrophobic Barrier Pen - Related Videos
03:22
Calcium-Dependent Hydrophobic Interaction Chromatography: A Technique to Purify Calcium-Binding Proteins Based on Hydrophobic Interactions
0 Views •
2025
In this video, we demonstrate the purification of calcium-binding protein from a dialyzed cell lysate through calcium-dependent hydrophobic interaction chromatography. The calcium-binding proteins expose a hydrophobic region upon binding with calcium, facilitating interaction with a hydrophobic group on resin. Later these proteins are eluted using calcium chelator EDTA that reverses the interaction.
03:46
Butyl-Dependant Hydrophobic Interaction Liquid Chromatography: A Technique to Characterize Antibody-Drug Conjugates Based on Hydrophobic Interactions
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2025
This video demonstrates the hydrophobic interaction liquid chromatography-based characterization of antibody-drug conjugates. Using a butyl ligand-based chromatography column, the antibody-drug conjugates are characterized via hydrophobic interactions with the ligand.
06:30
Noninvasive, In-pen Approach Test for Laboratory-housed Pigs
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Cited by 3 •
2019
This protocol describes a new behavioral test—the human approach test in the pigs' home pen—to detect functional deficits in laboratory pigs after subconcussive traumatic brain injury.
11:16
Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
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Cited by 23 •
2012
This article will describe the procedure for synthesizing a hydrophobically modified Nafion enzyme immobilization membrane and how to immobilize proteins and/or enzymes within the membrane and test their specific activity.
02:08
A Hydrophobic Tissue Clearing Method for a Rat Brain Tissue Sample
0 Views •
2025
Source: Kirchner, K.N., et al., A Hydrophobic Tissue Clearing Method for Rat Brain Tissue. J. Vis. Exp. (2020).This video demonstrates the method of hydrophobic tissue clearing of a rat brain tissue sample. The sample is sequentially dehydrated in methanol, treated with a dichloromethane and methanol mixture to dissolve lipids and pigments, and transferred to dibenzyl ether for complete tissue clearing, enabling detailed imaging without thin sectioning.
FAQs
The ink creates a water-repellent film that resists aqueous solutions, limiting buffers, stains, and antibody reagents to the marked area. This physical separation reduces unintended spreading and reagent mixing across the slide. Better containment can improve assay consistency while using smaller reagent volumes for tissue sections or cell samples.
A boundary defines the region available for liquid handling around a tissue section or cell sample. When the marked area appropriately encloses the sample, solutions can remain localized during staining steps instead of dispersing over the slide. This supports controlled exposure to reagents and helps maintain distinct assay conditions across neighboring regions.
The hydrophobic boundaries allow multiple samples or conditions to occupy different regions on one microscope slide while keeping their small-volume reagents separated. This arrangement can make side-by-side staining comparisons more practical and reduce cross-contamination from spreading liquids. In cancer research, such organization helps evaluate differences in tumor markers or cellular features.
Researchers first draw a water-repellent boundary around the tissue section or cell sample on the microscope slide. They then apply aqueous buffers, stains, or antibody reagents within the enclosed region and carry out the relevant staining workflow. Keeping liquids inside the marked area helps preserve localized treatment and limits unnecessary reagent loss.
The marked region is intended to contain aqueous solutions used with tissue sections or cell samples, including buffers, stains, and antibody reagents. These materials support workflows such as immunohistochemistry and immunofluorescence. Localizing them around the sample helps researchers expose the relevant material to staining reagents without allowing solutions to spread broadly across the slide.
It is useful when researchers need to visualize tumor markers or cellular features in tissue sections or cell samples. The containment provided by the marked region supports immunohistochemistry, immunofluorescence, and related staining workflows. These applications can help connect observed molecular patterns with cancer biology and studies examining treatment-related effects.