Mesothelial Cells
Mesothelial Cells - Related Videos
04:36
Mesothelial Cell Isolation: A Technique to Isolate Primary Mesothelial Cells From Human Omentum Tissue Specimen
0 Views •
2023
This video describes the protocol to isolate primary human mesothelial cells from the human omentum specimen. The mesothelial cells can be used to establish an in vitro ovarian cancer model to investigate ovarian cancer cell adhesion, invasion, and proliferation.
04:34
Mesothelial Clearance Assay: An In Vitro Technique to Quantify the Invasion Ability of Ovarian Cancer Spheroids into Mesothelial Cell Monolayers
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2023
This video describes a technique to quantitatively measure the interaction of ovarian cancer spheroids with mesothelial cells, using fluorescently labeled cells and time-lapse video microscopy. This in vitro assay models the interaction between metastasizing ovarian cancer cell spheroids and mesothelial cells lining the peritoneal cavity in vivo.
08:54
In vitro Mesothelial Clearance Assay that Models the Early Steps of Ovarian Cancer Metastasis
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Cited by 47 •
2012
The mesothelial clearance assay described here takes advantage of fluorescently labeled cells and time-lapse video microscopy to visualize and quantitatively measure the interactions of ovarian cancer multicellular spheroids and mesothelial cell monolayers. This assay models the early steps of ovarian cancer metastasis.
14:25
Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time
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Cited by 21 •
2014
Ovarian cancer cell invasion into the mesothelial lining of the peritoneum is a dynamic process over time. Utilizing a real time analyzer, the invasive capacity of ovarian cancer cells in a spheroid-mesothelial cell co-culture model can be quantified over prolonged time periods, providing insights into factors regulating the metastatic process.
04:17
Proximal Culture System: An In Vitro Culture Technique to Study Paracrine Signaling Between Cells
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2023
In this video, we develop a proximal co-culture model of ovarian cancer cells and primary human mesothelial cells. This physiologically relevant model allows the study of paracrine signaling, where the localized concentrations of the secreted factors are maintained while preventing direct cellular contact.
FAQs
Selective permeability allows mesothelial layers to function as controlled barriers around organs and body cavities rather than as completely sealed surfaces. This barrier property helps maintain separation between tissue compartments while permitting regulated interactions with surrounding fluids and signals. In cancer research, changes in this barrier are relevant because they may influence how tumor cells contact, attach to, or move across serosal surfaces.
Injury or inflammatory signals can activate mesothelial cells and stimulate repair-related responses. These cells may undergo phenotypic changes as the damaged surface is restored, altering their behavior and interactions with nearby tissues. This responsiveness matters in cancer research because repair-associated changes can affect the local environment that circulating tumor cells encounter during adhesion, invasion, and dissemination.
Epithelial-to-mesenchymal transition, or EMT, describes a shift in cellular phenotype that can change how mesothelial cells behave and interact with surrounding tissue. Its relevance comes from the ability of injury or inflammatory signals to stimulate such changes. Researchers therefore examine EMT as a possible link between mesothelial responses, tissue remodeling, and conditions that may support tumor invasion.
Mesothelial cells are studied in two related cancer contexts: they may represent potential cells of origin for mesothelioma, and they can act as active participants in the tumor microenvironment. These roles are distinct but complementary. One concerns how a serosal cancer may arise, while the other concerns how surrounding cells influence tumor behavior, progression, and response to treatment.
Researchers examine how circulating tumor cells interact with mesothelial surfaces, particularly whether those interactions promote adhesion and invasion. Such contacts may influence the ability of tumor cells to establish themselves within serosal tissues and contribute to metastatic spread. Studying these interactions helps connect cellular behavior at tissue surfaces with broader patterns of cancer dissemination.
Their location around the pleura, peritoneum, and pericardium places mesothelial cells at sites where tumor cells may encounter protective tissue surfaces. Because these cells can affect adhesion, invasion, metastasis, and treatment response, they provide a way to study the tissue context of serosal cancers. This perspective extends cancer research beyond tumor cells alone to include their surrounding cellular environment.