Mobile Phase Reservoirs
Mobile Phase Reservoirs - Related Videos
08:02
Reservoir Condition Pore-scale Imaging of Multiple Fluid Phases Using X-ray Microtomography
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Cited by 28 •
2015
We present a methodology for the imaging of multiple fluid phases at reservoir conditions by the use of x-ray microtomography. We show some representative results of capillary trapping in a carbonate rock sample.
01:30
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Veins, while chiefly responsible for circulating blood back to the heart, also function as storage vessels for blood. They house approximately 64 percent of the body's total blood volume, a feat made possible by their high capacitance—the inherent ability to expand and accommodate large volumes of blood, even under low pressure. The large diameter and thin walls of veins augment their distensibility, significantly more so than arteries, due to their classification as capacitance vessels. When...
06:57
Electrophoretic Mobility Shift Assay (EMSA)
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2023
The electrophoretic mobility shift assay (EMSA) is a biochemical procedure used to elucidate binding between proteins and nucleic acids. In this assay a radiolabeled nucleic acid and test protein are mixed. Binding is determined via gel electrophoresis which separates components based on mass, charge, and conformation. This video shows the concepts of EMSA and a general procedure, including gel and protein preparation, binding, electrophoresis, and detection. Applications covered in this video...
01:26
Mobile Marketing
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As consumers increasingly rely on smartphones and other mobile devices for browsing, communication, and shopping, mobile marketing has become essential in today's digital world. Brands must adjust their strategies to engage users where they spend most of their time, which is on mobile screens. Understanding mobile user behavior is crucial for successful mobile marketing. Mobile users typically interact with their devices briefly and frequently throughout the day. They prefer content that is...
FAQs
Filtration and degassing prepare the solvent before it reaches the pump. Together, these practices help prevent contamination and air bubbles from entering the flow path. That preparation supports stable solvent delivery, consistent retention times, and reliable peak shapes, so chemical analyses can be compared more confidently across runs within a chromatography system.
Multiple reservoirs allow a chromatography system to draw different solvents and vary their contributions during gradient elution. This changes the mobile-phase composition over the separation rather than relying on one fixed solvent mixture. The arrangement is useful when analytes require changing solvent conditions to achieve a separation, while controlled pumping helps maintain reproducible results.
Sealing matters because evaporation can change the solvent composition in a reservoir, potentially altering the conditions delivered through the system. Appropriate closure also helps limit contamination. These effects can influence retention times and peak shapes, so keeping reservoirs protected is a practical part of maintaining reliable chromatographic measurements.
Preparation should include choosing suitable containers, clearly labeling each solvent or solvent mixture, and cleaning reservoirs to reduce contamination. Analysts should also inspect the setup for conditions that could introduce air bubbles or allow evaporation, then connect the reservoirs to the tubing leading to the pump. These steps promote consistent operation and traceable solvent use.
During operation, the pump draws solvent from the reservoir through tubing and delivers it at a controlled flow rate. Before pumping, the mobile phase may pass through filtration and degassing steps. When several reservoirs are connected, the system can draw different solvents for gradient elution. This workflow links solvent preparation with controlled transport and separation performance.
They support reproducible chromatography in pharmaceutical analysis, environmental testing, and research chemistry. In each setting, contamination, evaporation, or bubbles can compromise solvent consistency and affect retention times or peak shapes. Careful reservoir practices therefore contribute to dependable chemical analysis, whether the goal is routine testing, environmental measurement, or investigative laboratory work.