Photoluminescence Imaging

Photoluminescence imaging is an optical method that maps light emitted by a material after it absorbs energy, revealing where chemical or physical properties vary across a sample. During measurement, excitation light promotes electrons to higher-energy states, and their return to lower-energy states produces fluorescence or phosphorescence that a camera records as spatially resolved intensity, color, or lifetime. In chemistry, this approach helps visualize molecular distributions, reaction progress, defects, and compositional changes in solutions, surfaces, and solid materials. Combining emission images with spectroscopy or time-resolved measurements can clarify energy-transfer pathways and support the development of sensors, catalysts, optoelectronic materials, and analytical assays.

Photoluminescence Imaging - Related Videos

Education

JoVE Core - Analytical Chemistry

Photoluminescence: Applications

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2024

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

Photoluminescence: Fluorescence and Phosphorescence

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2024

Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds. A pair of electrons in a...

Research

JoVE Journal - Chemistry

Close-Space Sublimation-Deposited Ultra-Thin CdSeTe/CdTe Solar Cells for Enhanced Short-Circuit Current Density and Photoluminescence

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Cited by 6 •

2020

This work describes the complete fabrication process of thin absorber cadmium selenium telluride/cadmium telluride photovoltaic devices for enhanced efficiency. The process utilizes an automated in-line vacuum system for close-space sublimation deposition that is scalable, from fabrication of small area research devices as well as large-scale modules.

Enhanced Photoluminescence of Curcuma longa Extracts via Chitosan-Mediated Energy Transfer for Textile Authentication Applications

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Cited by 1 •

2023

Photoluminescence is one of the most effective authentication mechanisms being used today. Utilizing and enhancing naturally sourced materials with inherent photoluminescent properties and incorporating them into fabric substrates can lead to development of green, sustainable, and functional textiles for smart applications.

Research

JoVE Journal - Bioengineering
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Hybrid µCT-FMT imaging and image analysis

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Cited by 34 •

2015

We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.

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