Plant Callus Culture
Plant Callus Culture - Related Videos
02:57
Plant Tissue Culture
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2020
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species. Plant tissue culture depends on the ability of plant tissue to give rise to an entire new plant when provided with a growth medium and appropriate environment. This ability of plant cells or tissues is termed ‘totipotency.’ The fundamental steps...
03:03
Preparation of Agrobacterium Culture for Gene Delivery into Plant Cells
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2026
Source: Shi, J., et al., Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens. J. Vis. Exp. (2020)This video demonstrates the procedure for preparing an Agrobacterium tumefaciens culture by inducing virulence gene expression with acetosyringone, followed by antibiotic removal through washing and resuspension, to generate a transformation-ready suspension for gene delivery into plant tissues.
10:22
Transmitting Plant Viruses Using Whiteflies
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Cited by 41 •
2013
Improving our understanding of and our ability to manage many of the insect-transmitted plant viruses requires the use of the vector. Insect transmission of plant viruses is a tritrophic interaction, and as such requires the manipulation of insects, virus, and plant. Vectors must be reared in large numbers and manipulated in such a way as to insure high rates of transmission to test plants. The basics of rearing and manipulating the whitefly, Bemisa tabaci (Hemiptera: Aleyrodidae), a vector of...
13:37
Generation of Composite Plants in Medicago truncatula used for Nodulation Assays
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Cited by 7 •
2011
We demonstrate how hairy root composite plants can be used to study plant-rhizobium interactions and nodulation in the difficult-to-transform species Medicago truncatula.
08:45
Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants
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Cited by 23 •
2009
A cell death-based assay for PTI in Nicotiana benthamiana plants is described.
FAQs
Balanced concentrations in nutrient medium support dedifferentiation, cell division, and callus formation. Changing the hormone conditions can redirect subsequent development toward roots, shoots, or complete plants. This hormonal control makes the culture useful not only for multiplying plant material, but also for studying how developmental outcomes change under controlled laboratory conditions.
Dedifferentiation allows previously specialized cells in an explant to return to a dividing state and produce callus. Because the resulting tissue can later respond to different hormone conditions, it provides an intermediate stage between the original plant tissue and regenerated roots, shoots, or whole plants. This flexibility underlies both propagation and experimental tissue selection.
Sterile laboratory conditions help maintain the culture as a defined plant-cell system, while controlled conditions make it easier to relate observed changes to drought, salinity, pollutants, or other stresses. Researchers can therefore examine stress effects on callus and compare cell-line behavior under controlled conditions, supporting more focused environmental investigations.
A supported workflow begins with an explant placed on a nutrient medium containing balanced auxin and cytokinin concentrations. Cells then dedifferentiate, divide, and form callus under sterile, controlled conditions. If regeneration is desired, researchers change the hormone conditions to encourage roots, shoots, or whole plants. The resulting material can support propagation, conservation, or stress studies.
The technique is useful when researchers need rapid clonal propagation or want to help conserve rare plant species. Its ability to produce regenerated plants from callus provides a tissue-culture route for multiplying plant material, while laboratory cultures offer a controlled resource for conservation-related work. It can also generate genetically variable material for separate research purposes.
Researchers can use callus cultures to investigate plant responses to drought, salinity, pollutants, and other stresses in a controlled system. The same platform supports selection of tolerant cell lines, allowing environmental research to focus on differences in how cultured plant cells respond to stress conditions. This makes the method relevant to controlled studies of plant stress tolerance.