Wax Embedding Technique
Wax Embedding Technique - Related Videos
02:43
Deparaffinization and Rehydration of Tissue Sections: A Two-step Procedure to Remove Paraffin Wax from FFPE Tissue Slides Followed by Tissue Rehydration
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2023
In this video, we demonstrate the processing of FFPE or formalin-fixed paraffin-embedded sections obtained from gastrointestinal tumor tissue. The deparaffinization and rehydration steps allow the processing of the tissue specimen for subsequent analytical applications.
09:17
Minimally Invasive Muscle Embedding (MIME) - A Novel Experimental Technique to Facilitate Donor-Cell-Mediated Myogenesis
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Cited by 3 •
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We describe a novel experimental technique that we call Minimally Invasive Muscle Embedding (MIME), which is based on the evidence that skeletal muscle tissue contains viable myogenic cells that can facilitate donor-cell-mediated myogenesis when implanted into a host muscle.
02:59
Embedding 3D Spheroids in Agarose Gel Drops: A Technique to Preserve Spheroids for Downstream Analysis
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This video describes the technique to embed 3D spheroids in agarose gel drops for preservation. The embedded 3D spheroids can be further used for downstream analysis.
07:46
Data Acquisition Protocol for Determining Embedded Sensitivity Functions
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The data acquisition procedure for determining embedded sensitivity functions is described. Data is acquired and representative results are shown for a residential scale wind turbine blade.
07:24
In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples
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Cited by 5 •
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The goal of this protocol is to perform in situ hybridization on adult coral samples that have been embedded in paraffin and sectioned onto glass slides. This is a qualitative method used to visualize the spatial expression of an RNA anti-sense probe in paraffin-embedded tissues.
FAQs
Dehydration removes water from the biological specimen before clearing and wax infiltration. This preparation is necessary because the later stages replace the specimen’s original water content with materials that support formation of a stable wax block. Effective water removal helps preserve delicate structures during processing, allowing them to remain suitable for thin-section microscopy and detailed cellular examination.
The clearing agent acts as an intermediate between dehydration and wax infiltration, preparing the specimen for contact with molten paraffin wax. The wax then penetrates the processed material and occupies the spaces left by the earlier treatment. Together, these stages transform a water-containing specimen into one supported by a firm matrix that can later be sectioned.
Cooling converts the infiltrated molten wax into a solid block that stabilizes the specimen for sectioning. This change in physical state is important because the supported material must retain its organization while thin sections are produced for microscopy. The resulting block provides a consistent form for examining structures that might otherwise be difficult to handle or preserve.
Environmental conditions can produce cellular or structural changes in plants, aquatic organisms, and other biological samples. Wax embedding preserves these tissues in a form suitable for microscopic evaluation, allowing researchers to examine changes associated with pollutants or changing conditions. The preserved sections help connect visible tissue-level effects with the environmental stress affecting the organism or sample.
Processing follows an ordered sequence: first remove water through dehydration, then treat the specimen with a clearing agent, and next infiltrate it with molten paraffin wax. After infiltration, cool the material so the wax solidifies into a block. Maintaining this sequence is important because each stage prepares the sample for the next form of support and examination.
The approach can support microscopic evaluation of plant tissues, aquatic organisms, and other biological samples relevant to environmental research. Its value is greatest when investigators need to examine preserved internal or cellular structures after exposure to pollutants or changing environmental conditions. The resulting sections provide a practical basis for comparing tissue organization and identifying structural responses.
Microscopy of the sections can reveal cellular or structural changes within preserved biological material. In environmental research, those observations can be related to exposure to pollutants or to changing conditions, helping investigators assess how stress affects plants, aquatic organisms, or other samples. The technique therefore supplies structural evidence that complements broader evaluations of environmental impact.