Li-cor Odyssey Scanner
Li-cor Odyssey Scanner - Related Videos
08:25
Using Flatbed Scanners to Collect High-resolution Time-lapsed Images of the Arabidopsis Root Gravitropic Response
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Cited by 6 •
2014
This protocol describes a process for rapid collection of images of Arabidopsis seedlings responding to a gravity stimulus using commercially-available flatbed scanners. The method allows for inexpensive, high-volume capture of high-resolution images amenable for downstream analysis algorithms.
07:01
Frequency Mixing Magnetic Detection Scanner for Imaging Magnetic Particles in Planar Samples
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Cited by 5 •
2016
A scanner for imaging magnetic particles in planar samples was developed using the planar frequency mixing magnetic detection technique. The magnetic intermodulation product response from the nonlinear nonhysteretic magnetization of the particles is recorded upon a two-frequency excitation. It can be used to take 2D images of thin biological samples.
01:13
meta-Directing Deactivators: –NO2, –CN, –CHO, –CO2R, –COR, –CO2H
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2023
All meta-directing substituents are deactivating groups. These substituents withdraw electrons from the aromatic ring, making the ring less reactive toward electrophilic substitution. For example, the nitration of nitrobenzene is 100,000 times slower than that of benzene because of the deactivating effect of the nitro group. The first step in an electrophilic aromatic substitution is the addition of an electrophile to form a resonance-stabilized carbocation. The energy diagrams for the...
10:05
High-throughput Detection Method for Influenza Virus
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Cited by 8 •
2012
This method describes the use of Infrared dye based imaging system for detection of H1N1 in bronchioalveolar lavage (BAL) fluid of infected mice at a high sensitivity. This methodology can be performed in a 96- or 384-well plate, requires <10 μl volume of test material and has the potential for concurrent screening of multiple pathogens.
08:36
Multi-Tracer Studies of Brain Oxygen and Glucose Metabolism Using a Time-of-Flight Positron Emission Tomography-Computed Tomography Scanner
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Cited by 2 •
2024
Quantitative measurements of oxygen and glucose metabolism by PET are established technologies, but details of practical protocols are sparsely described in the literature. This paper presents a practical protocol successfully implemented on a state-of-the-art positron emission tomography-computed tomography scanner.
FAQs
Samples carry labels that respond to infrared excitation, and the scanner records the fluorescence emitted from those labels. Because detection focuses on compatible near-infrared signals, naturally fluorescent material in the sample contributes less interference than approaches affected by stronger background autofluorescence. This improves separation of target-associated signal from background and supports more reliable measurement of biomolecular abundance.
Multiplexing depends on using compatible labels that produce distinguishable infrared fluorescence signals. The scanner can then detect multiple targets in the same sample rather than requiring separate imaging measurements for each one. In an immunology experiment, this allows a pathogen-associated target and a host-response target to be evaluated together, supporting direct comparison within one analysis.
Quantitative imaging converts fluorescence signal into measurements that can be compared across samples, whereas qualitative imaging mainly indicates whether a signal is present or visibly different. In infection studies, this distinction helps characterize changes in pathogen or host-response proteins and assess whether experimental treatments alter those measurements. It also supports more consistent comparisons than visual inspection alone.
A researcher first prepares samples containing compatible fluorescent labels, places them in the imaging system, and scans them with infrared excitation light. The resulting emission signals are used to detect and quantify labeled proteins, nucleic acids, or other biomolecules. Consistent scanning then enables comparisons among experimental samples and supports quantitative analysis of target-associated fluorescence.
In these formats, fluorescently labeled assay components generate signals associated with particular proteins or other targets. The scanner captures those signals so researchers can compare target levels across samples quantitatively rather than relying only on visual band or assay differences. This is useful for measuring immune signaling and infection-related protein changes in immunology experiments.
Researchers can compare immune signaling between conditions, track changes in proteins associated with infection, and examine how experimental treatments influence those changes. When pathogen and host-response measurements are obtained through compatible multiplexed analysis, the results can place infection-related and immune alterations in the same experimental context, supporting clearer interpretation of their relationship.