Lymphatic Vasculature Imaging
Lymphatic Vasculature Imaging - Related Videos
09:52
Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
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Cited by 25 •
2013
Recently developed imaging techniques using near-infrared fluorescence (NIRF) may help elucidate the role the lymphatic system plays in cancer metastasis, immune response, wound repair, and other lymphatic-associated diseases.
10:05
Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy
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Cited by 12 •
2020
A protocol is presented combining tissue clearing with light sheet fluorescence microscopy (LSFM) to obtain three-dimensional and cellular resolution images of the lymphatic vessels and lymph nodes (LNs) collecting the cerebrospinal fluid (CSF) and spinal epidural fluid.
09:36
The Assessment of Lymphatic Drainage Using ICG Near-Infrared Imaging and Ultrasound After Fracture
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2026
This protocol assesses lymphatic drainage after fracture by combining indocyanine green(ICG) near-infrared imaging with ultrasound to quantify plantar ICG clearance and popliteal lymph node enlargement in mice.
08:08
Measurement of Cytosolic Ca2+ in Isolated Contractile Lymphatics
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Cited by 24 •
2011
We introduce an approach to evaluate the cytosolic Ca2+ concentration in isolated lymphatics to study Ca2+-dependent and Ca2+-sensitizing mechanisms of lymphatic smooth muscle contraction.
09:53
Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
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Cited by 19 •
2011
We introduce a whole-mount immunohistochemistry and laser scanning confocal microscopy with multiple labelling for analyzing intricate vascular network formation in mouse embryonic limb skin.
FAQs
Lymph-targeted tracers, fluorescent labels, and contrast agents make lymphatic transport visible because they enter lymph and expose where fluid moves. When paired with anatomical imaging, they can relate a visible vessel pattern to drainage and transport rather than showing structure alone. This combination is useful for examining how immune cells, antigens, or pathogens travel through living tissues under defined conditions.
Anatomical imaging and lymph-targeted labels provide complementary information. Anatomical imaging identifies vessel structure, while labels or contrast agents enter lymph and reveal drainage and fluid movement. Examining both dimensions helps distinguish an anatomical change from a transport change, which matters when studying inflammation, infection, or immune trafficking.
Inflammation or infection can alter lymphatic vessel organization and function, making remodeling an important imaging outcome. Lymphatic vasculature imaging can document these changes while also measuring associated fluid transport. In immunology and infection studies, such measurements help connect lymphatic dysfunction with impaired host defense and provide a way to examine disease progression in affected tissues.
A basic workflow starts by selecting an anatomical imaging approach and a lymph-targeted tracer, fluorescent label, or contrast agent. The imaging material enters lymph, and the resulting signal is used to examine vessel structure, drainage, or transport in living tissue. Keeping conditions defined is important because it makes comparisons of lymphatic behavior interpretable.
One major application is following antigen delivery to lymph nodes. Imaging can show how antigen-related material moves through lymphatic pathways and reaches lymph nodes, supporting analysis of immune transport. The same strategy can help investigate immune-cell or pathogen movement, allowing researchers to relate lymphatic trafficking to immune responses during immunology and infection studies.
Quantitative measurements extend the method beyond visual documentation. By assessing vessel structure, drainage, and fluid movement, researchers can compare lymphatic function during infection-associated remodeling or after therapeutic intervention. These outcomes may reveal whether an intervention changes lymphatic behavior, clarify links between dysfunction and impaired host defense, and improve interpretation of disease progression in immunology and infection research.