Neutrophil Fluorescence Tracking
Neutrophil Fluorescence Tracking - Related Videos
15:01
Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy
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Cited by 21 •
2011
We describe a protocol of brightfield intravital microscopy for measuring dynamic neutrophil-endothelial cell interactions during neutrophil recruitment in response to the source of a neutrophil chemoattractant in vivo. Neutrophil intraluminal crawling, transendothelial migration and chemotaxis in mouse cremaster muscle tissue are visualized with time-lapsed video photography and tracked with ImageJ.
06:45
A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
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Cited by 8 •
2016
Neutrophil extracellular traps (NETs) are networks of DNA, histones and neutrophil proteins. Although a component of the innate immune response, NETs are implicated in autoimmunity and thrombosis. This protocol describes a simple method for canine neutrophil isolation and quantification of NETs using a microplate fluorescence assay.
02:59
An In Vitro Assay for Measuring Neutrophil Serine Protease Activity Using a Fluorescent Reporter
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2025
This video demonstrates an in vitro assay to quantify neutrophil serine protease activity in sputum samples. The sample containing secreted protease is incubated with a fluorescent reporter bearing a recognition motif. Cleavage of the motif by the protease enables individual fluorescence emission by the donor and acceptor fluorophore of the reporter, indicating protease activity in the sample.
08:39
Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy
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2025
This article provides a detailed description of how to create samples for single-protein tracking in solid-supported lipid bilayers. It also explains a straightforward fluorescence microscope with single-molecule sensitivity and a fast frame rate. Finally, we outline the procedure for extracting single-protein trajectories.
04:32
Induction and Visualization of Neutrophil Extracellular Traps in Pre-Labeled Neutrophils
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2025
This video demonstrates the induction and visualization of neutrophil extracellular traps or NETs in pre-labeled neutrophils using patient serum containing anti-neutrophil cytoplasmic antibodies. Furthermore, using an impermeable DNA dye and fluorescence microscopy enables the visualization of NETs.
FAQs
Recruitment and accumulation represent different features of the inflammatory response. Recruitment concerns how neutrophils reach a damaged or infected location, whereas accumulation reflects their increasing presence within that area. By examining fluorescent cells across successive images, investigators can separate changes in arrival and distribution from the overall buildup of neutrophils at the site.
Time-lapse imaging adds a temporal dimension that a single image cannot provide. It shows how neutrophil behavior changes during inflammation or infection, including movement toward affected tissue, persistence at the site, and interactions with pathogens or infected regions. These sequential observations connect cell location with the timing of an immune response.
Fluorescent dyes and reporters provide the visual signal needed to identify neutrophils during imaging. Once labeled, the cells can be followed against surrounding tissues, allowing their positions and changing distributions to be measured over time. This labeling step therefore links individual cellular visibility with analysis of migration, recruitment, accumulation, and tissue interactions.
A basic workflow includes labeling neutrophils with a fluorescent dye or reporter, positioning the relevant tissue or infection model for fluorescence microscopy, and acquiring images over time when dynamic behavior is important. The resulting image sequence can then be examined for cell migration, recruitment, accumulation, and interactions with infected tissues or pathogens.
Investigators can apply the method when they need to connect neutrophil behavior with host defense or inflammation. Imaging sites of tissue damage, infection, or pathogen exposure helps reveal how these cells reach affected areas and coordinate antimicrobial responses. The same measurements can also support studies of inflammatory disease, tissue injury, and repair.
The approach produces spatial and temporal information about where neutrophils are located and how their behavior changes during an immune response. Such measurements can clarify whether neutrophil activity is associated with antimicrobial defense, tissue injury, or repair. Consequently, the method can help evaluate therapeutic strategies intended to modify neutrophil activity in disease.