Plant Bacterial Interaction

Plant-bacterial interaction is the relationship between plants and bacterial communities, ranging from beneficial associations that promote growth to infections that cause disease, and it is central to plant immunity and microbial ecology. Plants detect conserved bacterial molecules, such as flagellin or lipopolysaccharides, through pattern-recognition receptors, triggering pattern-triggered immunity that includes signaling, production of reactive oxygen species, and defense gene activation. Pathogenic bacteria can deliver effector proteins that suppress these defenses, whereas plants may recognize specific effectors and mount a stronger effector-triggered response. Understanding these exchanges supports research on crop disease resistance, biological control, microbiome engineering, and sustainable strategies for improving plant health and food security.

Plant Bacterial Interaction - Related Videos

Research

JoVE EoE - Bacterial Growth and Techniques

Treatment of Bacteria with Plant Root Exudates to Emulate In Vitro Plant-Microbe Interactions

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2025

Source: Yi, Y., et al. Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates. J. Vis. Exp. (2018)This video demonstrates the preparation and treatment of Bacillus mycoides cultures to study plant-microbe interactions. It outlines the steps from streaking a stock culture and incubating it in liquid medium to exposing the bacteria to plant root exudates under agitation. The process culminates in harvesting and flash-freezing the bacterial cells for...

Monitoring Bacterial Infection and Effector Protein Delivery in Plant Cells

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2025

Source: Lee, H. et. al., Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection. J. Vis. Exp. (2018)This video demonstrates a method for monitoring bacterial infection and effector protein delivery in plant cells using split superfolder green fluorescent protein (sfGFP) complementation combined with confocal microscopy. The approach enables precise visualization of effector translocation into the host cell cytosol.

Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation (BiFC) System

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Cited by 30 •

2011

We have developed a technique to test protein-protein interactions in plant. A yellow fluorescent protein (YFP) is split into two non-overlapping fragments. Each fragment is cloned in-frame to a gene of interest via Gateway system, enabling expression of fusion proteins. Reconstitution of YFP signal only occurs when the inquest proteins interact.

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling

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Cited by 6 •

2017

The described hydroponic cocultivation system supports intact plants with metal mesh screens and cocultivates them with bacteria. Plant tissue, bacteria, and secreted molecules can then be separately harvested for downstream analyses, simultaneously allowing for the molecular responses of both plant hosts and interacting microbes or microbiomes to be investigated.

Visualization of Single-Cell Bacterial Interactions

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2025

Source: Yarringto, K. D., et al. Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions. J. Vis. Exp. (2020)This video demonstrates the preparation of a coculture system using motile and non-motile bacteria under an agarose pad for live-cell imaging. It shows how motile bacteria detect chemical attractants and invade non-motile colonies, revealing bacterial interactions.

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