High-resolution Magic Angle Spinning
High-resolution Magic Angle Spinning - Related Videos
07:33
Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning
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Cited by 5 •
2010
This technique enables the use of high-resolution magic angle spinning proton MR spectroscopy (HRMAS 1H-MRS) for molecular characterization of live Drosophila melanogaster with a conventional 14.1 tesla spectrometer equipped with an HRMAS probe.
08:55
High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
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Cited by 11 •
2020
The molecular structures and dynamics of solids, liquids, gases, and mixtures are of critical interest to diverse scientific fields. High-temperature, high-pressure in situ MAS NMR enables detection of the chemical environment of constituents in mixed phase systems under tightly controlled chemical environments.
06:42
Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
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Cited by 15 •
2020
Presented here is a protocol for cryogenic transfer of frozen samples into the dynamic nuclear polarization (DNP) magic angle spinning (MAS) nuclear magnetic resonance (NMR) probe. The protocol includes directions for rotor storage prior to the experiment and directions for viability measurements before and after the experiment.
08:41
Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
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Cited by 11 •
2012
Lensfree optical tomography is a three-dimensional microscopy technique that offers a spatial resolution of <1 μm × <1 μm × <3 μm in x, y and z dimensions, respectively, over a large imaging-volume of 15-100 mm3, which can be particularly useful for integration with lab-on-a-chip platforms.
01:29
Magical Thinking
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2025
Magical thinking encompasses the belief in assumptions that defy logical reasoning yet appear intuitively convincing. It is a common psychological phenomenon that persists across various cultural and individual contexts. While these assumptions contradict empirical evidence and scientific laws, they often serve meaningful psychological roles in promoting emotional resilience and a sense of control, especially under stress or uncertainty.Thought-Action Fusion and the Law of SimilarityA key...
FAQs
Rapid spinning at the magic angle suppresses contributions from anisotropic interactions, which otherwise broaden resonances in tissue spectra. Reducing this broadening improves resolution and allows more molecular signals to be distinguished within an intact biological sample. This matters because HR-MAS can retain tissue context while producing spectra that are easier to interpret metabolically.
The 54.74° orientation relative to the magnetic field is selected because it averages anisotropic interactions during rapid sample rotation. This averaging reduces spectral broadening without requiring the tissue to be converted into a fully liquid preparation. As a result, the measurement combines improved spectral detail with preservation of the original tissue structure.
Direct analysis preserves the tissue as an intact biological sample while still generating spectra with solution-like characteristics. That combination provides molecular information without separating the sample into isolated components. For neuroscience studies, retaining tissue structure alongside metabolic signals helps connect detected amino acids, lipids, and neurotransmitter-related metabolites to the tissue being investigated.
A typical workflow uses an intact tissue sample with minimal preparation, positions it for nuclear magnetic resonance measurement, and rapidly spins it at the magic angle. The resulting spectrum is then examined for molecular signals and overall metabolic patterns. This workflow supports metabolic profiling while limiting preparation that could alter the original tissue composition.
HR-MAS can detect small-molecule signals from brain tissue, including amino acids, lipids, and metabolites related to neurotransmitters. The combined pattern of these signals forms a molecular fingerprint rather than a single measurement of one compound. Investigators can use that fingerprint to characterize metabolic features of the tissue and compare biological conditions.
In neuroscience, HR-MAS supports metabolic profiling across studies of brain development, neurological disease, tumor biology, and treatment responses. Researchers can examine molecular fingerprints in tissue with minimal sample preparation, then relate differences in amino acids, lipids, or neurotransmitter-related metabolites to the biological condition under study. This makes the technique useful across diverse tissue-based investigations.