Mouse Model Brain Imaging
Mouse Model Brain Imaging - Related Videos
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
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Cited by 59 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
06:43
Imaging Brain Damage Induced by Cerebral Hypoxia-Ischemia in a Mouse Model
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2025
Source: Ouyang, Y., et al. Simultaneous PET/MRI Imaging During Mouse Cerebral Hypoxia-ischemia. J. Vis. Exp. (2015)This video demonstrates a technique to assess cerebral hypoxia injury via simultaneous positron-emission tomography (PET) and magnetic resonance imaging (MRI). An anesthetized mouse undergoes common carotid artery (CCA) ligation, reducing blood flow to one brain hemisphere and predisposing it to hypoxia-induced injury. PET and MRI imaging evaluate glucose metabolism and structural...
04:19
A Mouse Model of Single and Repetitive Mild Traumatic Brain Injury
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Cited by 16 •
2017
Athletes absorb several hundred mild traumatic brain injuries (mTBI)/concussions every year; however, the consequence of these on the brain is poorly understood. Therefore, an animal model of single and repetitive mTBI that consistently replicates clinically relevant symptoms provides the means to advance the study of mTBI and concussion.
03:04
High-Resolution Three-Dimensional Confocal Imaging of the Blood-Brain Barrier in a Mouse Brain Section
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2025
Source: Villaseñor, R and Collin. L. High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis. J. Vis. Exp. (2017)This video demonstrates high-resolution 3D imaging of the blood-brain barrier (BBB) using confocal microscopy, showing antibody-stained mouse brain sections to analyze BBB components and intracellular structures, applicable for studying brain homeostasis, neurovascular interactions, and disease-related BBB...
04:58
Brain Metastasis Mouse Model: A Method to Generate Brain Metastasis Mouse Model by Intracarotid Cancer Cell Injection
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2025
This video demonstrates the establishment of a brain metastasis mouse model by intracarotid injection of fluorescently labeled cancer cells. The mouse model can be used to study the effects of novel therapeutics and the underlying physiological mechanisms.
FAQs
The modality should match the level of biology being measured. MRI is suited to mapping tissue properties, two-photon microscopy reveals fluorescence from labeled cells beneath a cranial window, and PET measures radiotracer distribution. This distinction allows investigators to choose structural, cellular, or distribution-based information rather than treating all imaging methods as interchangeable.
MRI maps properties of brain tissue, making it useful for examining anatomical changes associated with disease or injury. Its contribution is distinct from methods that visualize labeled cells or radiotracers, because the measurement is based on tissue characteristics. In neuroscience studies, these maps can help relate pathology to behavioral findings or treatment-associated changes.
Two-photon excitation produces fluorescence from labeled cells beneath a cranial window, linking imaging observations to specific cellular populations. This provides a cellular perspective that complements broader measurements from MRI or PET. When paired with genetic mouse models, the approach can help connect selected cells or neural circuits with physiological changes and disease-related processes.
A study begins by matching the imaging method to the question, such as tissue mapping, labeled-cell visualization, or radiotracer distribution. Researchers can then relate the imaging findings to a genetic mouse model and behavioral assays. Interpreting these sources together helps connect anatomy, cellular or physiological changes, behavior, and disease progression.
It is valuable when investigators need to connect changes in the living brain with disease progression or experimental intervention. The overview identifies neurodegeneration, stroke, tumors, and therapeutic responses as important applications. Imaging can provide a bridge between cellular neuroscience and organism-level disease studies, especially when combined with genetic models and behavioral assessment.
Each modality contributes a different type of evidence: MRI describes tissue properties, two-photon microscopy focuses on fluorescence from labeled cells, and PET indicates radiotracer distribution. Combining these views can relate structure, cellular activity or labeling, and tracer patterns within the same research context. Behavioral assays add functional relevance by connecting imaging findings with observed outcomes.