Myogenic Reactivity
Myogenic Reactivity - Related Videos
13:43
Fabrication of Myogenic Engineered Tissue Constructs
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Cited by 11 •
2009
Here, we demonstrate fabrication of collagen-based, tissue constructs containing skeletal myoblasts. These 3-D engineered constructs may be used to replace or repair tissues in vivo. For our purposes, we have designed these as an atrioventricular electrical conduit for the repair of complete heart block[1].
07:51
Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
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Cited by 32 •
2014
In vitro culture systems have proven indispensible to our understanding of vertebrate myogenesis. However, much remains to be learned about nonmammalian skeletal muscle development and growth, particularly in basal taxa. An efficient and robust protocol for isolating the adult stem cells of this tissue, the myogenic precursor cells (MPCs), and maintaining their self-renewal, proliferation, and differentiation in a primary culture setting allows for the identification of conserved and divergent...
10:48
Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography
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Cited by 24 •
2015
Pressure myography is used to assess vasoactivity of small arteries that develop sustained constriction when pressurized. This manuscript provides a detailed protocol to assess in isolated segments of small mesenteric arteries from rats, vasoactivity and the effect of intraluminal pressure on vascular diameter.
10:45
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
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Cited by 10 •
2017
Here, we describe a methodology to obtain a pure population of human myoblasts from adult muscle tissue. These cells are used to study in vitro skeletal muscle differentiation and, in particular, to study proteins involved in Ca2+ signaling.
09:08
Detecting Reactive Oxygen Species
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2023
Reactive oxygen species are chemically active, oxygen-derived molecules capable of oxidizing other molecules. Because of their reactive nature, there are many deleterious effects associated with unchecked ROS production, including structural damage to DNA and other biological molecules. However, ROS can also be mediators of physiological signaling. There is accumulating evidence that ROS play significant roles in everything from activation of transcription factors to the mediation of...
FAQs
An increase in intravascular pressure stretches the vascular smooth muscle within the vessel wall. This mechanical change is sensed by the muscle, which alters its membrane potential. The electrical change then promotes opening of voltage-dependent calcium channels, linking pressure detection to intracellular calcium regulation and ultimately to smooth muscle contraction.
Voltage-dependent calcium channels provide the connection between membrane-potential changes and contraction. After mechanical sensing alters the smooth muscle membrane potential, these channels open and influence intracellular calcium. Because calcium regulates contraction, channel activity determines how effectively a vessel converts a pressure-related mechanical signal into a change in vascular tone.
A fall in intravascular pressure reduces the stretch applied to the vascular wall and shifts the smooth muscle response toward relaxation. This pressure-dependent adjustment is the counterpart to constriction during increased pressure. Together, the opposing responses allow vascular tone to change with pressure rather than remaining fixed at one contractile state.
In cerebral vessels, pressure-sensitive changes in smooth muscle tone help stabilize blood flow when systemic arterial pressure changes. By constricting or relaxing in response to altered vessel-wall stretch, the vascular system can moderate the effect of pressure fluctuations on the brain. This supports a more consistent environment for neural tissue and neuronal function.
The response connects vascular mechanics with the conditions experienced by neural tissue. Studying it helps explain how cerebral vessels respond to systemic arterial pressure changes while supporting an appropriate environment for neuronal function. It therefore provides a vascular perspective on brain regulation, complementing investigations focused directly on neurons or neural signaling.
Impaired myogenic reactivity may signal cerebrovascular dysfunction because the vessels could respond less effectively to changes in systemic arterial pressure. Studying this impairment can help researchers examine whether pressure-related regulation of cerebral blood flow is compromised. The outcome is relevant to understanding how altered vascular control may affect the environment that supports neural tissue.