Neuroblastoma Mitochondria
Neuroblastoma Mitochondria - Related Videos
02:42
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This video demonstrates the procedure to assess mitochondrial calcium retention capacity by monitoring real-time changes in fluorescence as mitochondria uptake calcium. This assay is useful for studying mitochondrial function and calcium-related processes, including apoptosis and neurodegenerative diseases.
13:42
Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria
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Cited by 19 •
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A spectrofluorometric protocol for the measurement of the mitochondrial permeability transition pore opening in isolated mouse heart mitochondria is presented here. The assay involves the simultaneous measurement of mitochondria Ca2+ handling, mitochondrial membrane potential and mitochondrial volume. The procedure for obtaining high-quality and functional heart mitochondria is also described.
19:44
Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen
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Cited by 10 •
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We have synthesized a novel analogue of pancratistatin with comparable anti-cancer activity as native pancratistatin; interestingly, combinatory treatment with tamoxifen yielded a drastic enhancement in apoptotic and autophagic induction by mitochondrial targeting with minimal effect on noncancerous fibroblasts. Thus, JCTH-4 in combination with tamoxifen could provide a safe anti-cancer therapy.
10:39
Purification of Mitochondria from Yeast Cells
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Cited by 85 •
2009
We describe a rapid and effective method for purification of mitochondria from the yeast Saccharomyces cerevisiae. This method enables the high-yield isolation of pure mitochondria that are essentially free of contamination by other organelles and retain their structural and functional integrity after their purification.
07:48
Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds
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Cited by 9 •
2021
This paper lists the steps required to seed neuroblastoma cell lines on previously described three-dimensional collagen-based scaffolds, maintain cell growth for a predetermined timeframe, and retrieve scaffolds for several cell growth and cell behavior analyses and downstream applications, adaptable to satisfy a range of experimental aims.
FAQs
Their functions are linked rather than independent. Oxidative phosphorylation supplies ATP for cellular activity, while mitochondrial control of reactive oxygen species helps maintain redox balance. If mitochondrial pathways shift toward conditions that favor apoptosis, or programmed cell death, they can reduce tumor-cell survival. This connection makes mitochondrial activity relevant to both neuroblastoma growth and treatment response.
Altered mitochondria may help neuroblastoma cells meet the energy demands of rapid growth and tolerate cellular stress. Changes in function can also influence redox balance and pathways that control apoptosis. Together, these adaptations may support survival under unfavorable conditions and contribute to treatment resistance, making structural and functional abnormalities important features to investigate.
Mitochondrial redox regulation concerns the balance associated with reactive oxygen species, which are connected to both cellular stress and survival signaling. Excessive or poorly controlled changes may activate mitochondrial pathways leading to apoptosis, whereas effective regulation can support adaptation. Studying this balance helps explain how neuroblastoma cells persist while maintaining the metabolism required for growth.
A focused evaluation can examine mitochondrial structure, oxidative phosphorylation, ATP production, reactive oxygen species, redox balance, and pathways associated with apoptosis. Considering these features together is more informative than measuring energy production alone because altered tumor behavior may reflect coordinated changes in metabolism and cell-death control. The resulting profile can reveal candidate metabolic vulnerabilities.
Researchers can evaluate therapies that disrupt tumor bioenergetics, meaning the processes that provide usable cellular energy, or that restore programmed cell death. Mitochondrial measurements can help determine whether an intervention changes oxidative phosphorylation, ATP generation, redox balance, or apoptosis-related pathways. These outcomes connect a treatment's metabolic effect with its potential to reduce tumor-cell survival.
Neuroblastoma arises from developing cells of the sympathetic nervous system, so its mitochondrial biology links cancer research with the metabolism of neural development. Examining how organelles support growth, stress adaptation, and survival provides neuroscience context while also addressing cancer-specific vulnerabilities. This interdisciplinary perspective can help relate tumor behavior to the biology of the nervous system from which it develops.