Organ Bath Technique
Organ Bath Technique - Related Videos
03:08
Basic Organic Chemistry Techniques - Concepts
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2020
Glassware in Organic Chemistry There are standard pieces of glassware that are used in the organic chemistry lab. Beakers and Erlenmeyer flasks are typically used for simple mixing or to hold solvents but they should not be used to measure volume, unless only an approximate volume is needed. Glassware can be divided into the following categories: Volumetric Glassware – This includes glassware such as graduated cylinders, volumetric glass pipettes, and volumetric flasks. The purpose of these...
15:40
Basic Organic Chemistry Techniques - Student Protocol
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2020
Source: Lara Al Hariri and Ahmed Basabrain at the University of Massachusetts Amherst, MA, USA Synthesis of Benzoic AcidExpand In this lab, you'll learn several important techniques that you'll use throughout this class. This includes measuring the mass or volume of reactants and products, methods of heating and cooling reaction mixtures, proper selection and handling of glassware, and separating solids and liquids by vacuum filtration. First, you'll synthesize benzoic acid by protonating...
02:30
Basic Organic Chemistry Techniques - Instructor Prep
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2020
Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA Preparation of the LaboratoryExpand Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed. To set up for this lab experiment, wear the appropriate personal protective equipment, including a lab coat, chemical splash goggles, and gloves. Ensure that there are labeled waste containers for non-halogenated organic waste, hydrochloric...
09:05
Measurement of Smooth Muscle Function in the Isolated Tissue Bath-applications to Pharmacology Research
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Cited by 68 •
2015
This protocol describes the measurement of isometric contraction in an isolated smooth muscle preparation, using an isolated tissue bath system and computer-based data acquisition.
02:52
A Technique to Establish a Bacterial Infection Model in an Ex Vivo Organ Culture
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2025
This video demonstrates a method to establish an infection model in placental tissues to study infection propagation. An ex vivo placental organ culture is infected with Listeria monocytogenes, a facultative intracellular parasite. The bacteria invade extravillous trophoblasts (EVTs) to enter the placental tissue and then propagate to other cells via cell-to-cell spread.
FAQs
These conditions help maintain the isolated tissue in a controlled physiological environment during measurement. Oxygenation supports tissue viability, while temperature and pH provide defined experimental conditions for interpreting responses to drugs, neurotransmitters, or other stimuli. Keeping these variables controlled reduces environmental variation, making changes in contraction, relaxation, or electrical activity more directly attributable to the experimental manipulation.
A force transducer records mechanical changes such as contraction or relaxation, whereas another sensor can monitor electrical activity. The measured signal therefore depends on the tissue preparation and the response being investigated. In neuroscience, these recordings can reveal functional effects associated with receptor activity, synaptic signaling, or neurovascular and neuromuscular responses.
Researchers expose the tissue preparation to controlled experimental concentrations and compare the resulting changes in measured activity. This creates a concentration-response relationship that helps characterize how strongly a drug or neurotransmitter affects the preparation. Such measurements support pharmacological screening and provide a way to examine receptor function under defined ex vivo conditions.
An isolated preparation removes much of the complexity of a whole animal while preserving selected tissue responses. This narrower setting allows investigators to examine drug, neurotransmitter, receptor, or signaling effects under controlled conditions. The approach is especially useful when the goal is to relate a measured contraction, relaxation, or electrical response to a defined experimental manipulation.
A tissue or organ preparation is isolated, suspended in oxygenated physiological solution, and maintained at a defined temperature and pH. A force transducer or other sensor is then positioned to record the relevant response. Drugs, neurotransmitters, or physiological stimuli are applied as experimental manipulations, and the resulting mechanical or electrical changes are measured.
The method is useful when investigators need to study receptor function, synaptic signaling, or neurovascular and neuromuscular responses in an isolated preparation. It permits direct testing of defined drugs or neurotransmitters without whole-animal complexity. Researchers can therefore use it for mechanistic studies and pharmacological screening involving nervous system function.
Depending on the preparation and sensor, the experiment can provide measurements of contraction, relaxation, or electrical activity after a controlled stimulus or drug exposure. These outcomes can be compared across concentrations to characterize tissue responsiveness. In neuroscience, the resulting data help connect functional tissue behavior with receptor-mediated, synaptic, neurovascular, or neuromuscular mechanisms.