Laser Micro Machining

Laser micromachining is a precision manufacturing process that uses focused laser energy to cut, drill, pattern, or modify materials at micrometer-scale dimensions. The laser concentrates light into a small region, where controlled energy deposition causes localized melting, vaporization, ablation, or other material changes; beam position, intensity, wavelength, and pulse duration determine the result. In physics and engineering, the technique supports fabrication of microchannels, apertures, sensors, electronic components, and intricate surface structures across metals, polymers, ceramics, and other materials. Its noncontact operation and fine spatial control enable complex geometries, reduced mechanical stress, and rapid prototyping for research and advanced manufacturing.

Laser Micro Machining - Related Videos

Research

JoVE Journal - Bioengineering
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Quantitative Locomotion Study of Freely Swimming Micro-organisms Using Laser Diffraction

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Cited by 10 •

2012

Microscopic organisms like the free-swimming nematode C. elegans, live and behave in a complex three-dimensional environment. We report on a novel approach that provides analysis of C. elegans using diffraction patterns. This approach consists of tracking the temporal periodicity of diffraction patterns generated by directing laser light through a cuvette.

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

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Cited by 30 •

2017

Confocal fluorescence microscopy and laser micro-irradiation offer tools for inducing DNA damage and monitoring the response of DNA repair proteins in selected sub-nuclear areas. This technique has significantly advanced our knowledge of damage detection, signaling, and recruitment. This manuscript demonstrates these technologies to examine single and double strand break repair.

Research

JoVE Journal - Biology
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Laser Micro-Irradiation to Study DNA Recruitment During S Phase

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Cited by 6 •

2021

This protocol describes a non-invasive method to efficiently identify S-phase cells for downstream microscopy studies, such as measuring DNA repair protein recruitment by laser micro-irradiation.

Research

JoVE Journal - Genetics

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation

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Cited by 16 •

2018

Studying DNA damage repair kinetics requires a system to induce lesions at defined sub-nuclear regions. We describe a method to create localized double-stranded breaks using a laser-scanning confocal microscope equipped with a 405 nm laser and provide automated procedures to quantify the dynamics of repair factors at these lesions.

Micro-particle Image Velocimetry for Velocity Profile Measurements of Micro Blood Flows

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Cited by 12 •

2013

Micro-particle image velocimetry (μPIV) is used to visualize paired images of micro particles seeded in blood flows which are cross-correlated to give an accurate velocity profile. Shear rate, maximum velocity, velocity profile shape, and flow rate, each of which has clinical applications, can be derived from these measurements.

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